Human transferrin receptor

Expression of the receptor is assigned to chromosome 3

Caroline Enns, H. A. Suomalainen, J. E. Gebhardt, J. Schröder, H. H. Sussman

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Human chromosome 3 has been identified as responsible for expression of the transferrin receptor in mouse-human lymphocyte hybrids. The receptor was detected by immunoprecipitation with anti-human receptor antibody of 125I-labeled cells. This method also detected a similar 94,000-dalton protein in mouse cells. A radioimmunoassay developed for the human transferrin receptor measured 10% crossreactivity with the mouse protein. The two proteins were distinguished by NaDodSo4/polyacrylamide gel patterns of partial proteolytic digests of the immunoprecipitated proteins. Mouse-human hybrids were generated by fusing a mouse thymoma (BW5147) cell line to either concanavalin A- or pokeweed mitogen-activated human peripheral blood lymphocytes or a mouse myeloma (NS-1) to uncultured human peripheral blood lymphocytes. Each hybrid was karyotyped with respect to both mouse and human chromosomes. In every case, expression of the human transferrin receptor correlated only with human chromosome 3.

Original languageEnglish (US)
Pages (from-to)3241-3245
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number10 I
DOIs
StatePublished - 1982
Externally publishedYes

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Transferrin Receptors
Chromosomes, Human, Pair 3
Human Chromosomes
Lymphocytes
Proteins
Pokeweed Mitogens
Thymoma
Concanavalin A
Immunoprecipitation
Radioimmunoassay
Cell Line
Antibodies

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Human transferrin receptor : Expression of the receptor is assigned to chromosome 3. / Enns, Caroline; Suomalainen, H. A.; Gebhardt, J. E.; Schröder, J.; Sussman, H. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, No. 10 I, 1982, p. 3241-3245.

Research output: Contribution to journalArticle

Enns, Caroline ; Suomalainen, H. A. ; Gebhardt, J. E. ; Schröder, J. ; Sussman, H. H. / Human transferrin receptor : Expression of the receptor is assigned to chromosome 3. In: Proceedings of the National Academy of Sciences of the United States of America. 1982 ; Vol. 79, No. 10 I. pp. 3241-3245.
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