Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress

Marielle Gold, T. Eid, S. Smyk-Pearson, Y. Eberling, G. M. Swarbrick, S. M. Langley, Philip Streeter, Deborah Lewinsohn, David Lewinsohn

Research output: Contribution to journalArticle

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Abstract

Human mucosal-associated invariant T (MAIT) cells express the semi-invariant T-cell receptor (TCR) Vα7.2 and are restricted by the major histocompatibility complex-Ib molecule MR1. While MAIT cells share similarities with other innate T cells, the extent to which MAIT cells are innate and their capacity to adapt is unknown. We evaluated the function of Vα7.2 + T cells from the thymus, cord blood, and peripheral blood. Although antigen-inexperienced MAIT cells displayed a naïve phenotype, these had intrinsic effector capacity in response to Mycobacterium tuberculosis (Mtb)-infected cells. Vα7.2+ effector thymocytes contained signal joint TCR gene excision circles (sjTRECs) suggesting limited replication and thymic origin. In evaluating the capacity of Mtb-reactive MAIT cells to adapt, we found that those from the peripheral blood demonstrated a memory phenotype and had undergone substantial expansion, suggesting that they responded to antigenic stimulation. MAIT cells, an evolutionarily conserved T-cell subset that detects a variety of intracellular infections, share features of innate and adaptive immunity.

Original languageEnglish (US)
Pages (from-to)35-44
Number of pages10
JournalMucosal Immunology
Volume6
Issue number1
DOIs
StatePublished - Jan 2013

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Mycobacterium tuberculosis
T-Lymphocytes
Phenotype
T-Cell Receptor Genes
Replication Origin
T-Lymphocyte Subsets
Adaptive Immunity
Thymocytes
T-Cell Antigen Receptor
Major Histocompatibility Complex
Fetal Blood
Innate Immunity
Thymus Gland
Mucosal-Associated Invariant T Cells
Joints
Antigens
Infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. / Gold, Marielle; Eid, T.; Smyk-Pearson, S.; Eberling, Y.; Swarbrick, G. M.; Langley, S. M.; Streeter, Philip; Lewinsohn, Deborah; Lewinsohn, David.

In: Mucosal Immunology, Vol. 6, No. 1, 01.2013, p. 35-44.

Research output: Contribution to journalArticle

Gold, Marielle ; Eid, T. ; Smyk-Pearson, S. ; Eberling, Y. ; Swarbrick, G. M. ; Langley, S. M. ; Streeter, Philip ; Lewinsohn, Deborah ; Lewinsohn, David. / Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. In: Mucosal Immunology. 2013 ; Vol. 6, No. 1. pp. 35-44.
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