Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules

S. Windschüttl, C. Kampfer, C. Mayer, F. Flenkenthaler, T. Fröhlich, J. U. Schwarzer, F. M. Köhn, Henryk Urbanski, G. J. Arnold, A. Mayerhofer

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Male fertility depends on spermatogenesis, which takes place in the seminiferous tubules of the testis. This compartment is devoid of blood vessels, which are however found in the wall of the seminiferous tubules. Our proteomic study using cultured human testicular peritubular cells (HTPCs) i.e. the cells, which form this wall, revealed that they constitutively secrete pigment epithelium-derived factor, PEDF, which is known to exert anti-angiogenic actions. Immunohistochemistry supports its presence in vivo, in the human tubular wall. Co-culture studies and analysis of cell migration patterns showed that human endothelial cells (HUVECs) are repulsed by HTPCs. The factor involved is likely PEDF, as a PEDF-antiserum blocked the repulsing action. Thus testicular peritubular cells, via PEDF, may prevent vascularization of human seminiferous tubules. Dihydrotestosterone (DHT) increased PEDF (qPCR) in HTPCs, however PEDF expression in the testis of a non-human primate occurs before puberty. Thus PEDF could be involved in the establishment of the avascular nature of seminiferous tubules and after puberty androgens may further reinforce this feature. Testicular microvessels and blood flow are known to contribute to the spermatogonial stem cell niche. Hence HTPCs via control of testicular microvessels may contribute to the regulation of spermatogonial stem cells, as well.

    Original languageEnglish (US)
    Article number12820
    JournalScientific Reports
    Volume5
    DOIs
    StatePublished - Sep 3 2015

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    Seminiferous Tubules
    Puberty
    Microvessels
    Testis
    Stem Cell Niche
    pigment epithelium-derived factor
    Dihydrotestosterone
    Spermatogenesis
    Coculture Techniques
    Proteomics
    Primates
    Androgens
    Cell Movement
    Fertility
    Blood Vessels
    Immune Sera
    Stem Cells
    Endothelial Cells
    Immunohistochemistry

    ASJC Scopus subject areas

    • General

    Cite this

    Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules. / Windschüttl, S.; Kampfer, C.; Mayer, C.; Flenkenthaler, F.; Fröhlich, T.; Schwarzer, J. U.; Köhn, F. M.; Urbanski, Henryk; Arnold, G. J.; Mayerhofer, A.

    In: Scientific Reports, Vol. 5, 12820, 03.09.2015.

    Research output: Contribution to journalArticle

    Windschüttl, S, Kampfer, C, Mayer, C, Flenkenthaler, F, Fröhlich, T, Schwarzer, JU, Köhn, FM, Urbanski, H, Arnold, GJ & Mayerhofer, A 2015, 'Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules', Scientific Reports, vol. 5, 12820. https://doi.org/10.1038/srep12820
    Windschüttl, S. ; Kampfer, C. ; Mayer, C. ; Flenkenthaler, F. ; Fröhlich, T. ; Schwarzer, J. U. ; Köhn, F. M. ; Urbanski, Henryk ; Arnold, G. J. ; Mayerhofer, A. / Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules. In: Scientific Reports. 2015 ; Vol. 5.
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    AU - Fröhlich, T.

    AU - Schwarzer, J. U.

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