TY - JOUR
T1 - Human T lymphocyte response to myelin basic protein
T2 - Selection of T lymphocyte lines from mbp‐responsive donors
AU - Vandenbark, A. A.
AU - Chou, Y. K.
AU - Bourdette, D.
AU - Whitham, Ruth
AU - Chilgren, J.
AU - Chou, C. ‐H J.
AU - Konat, G.
AU - Hashim, G.
AU - Vainiene, M.
AU - Offner, H.
PY - 1989/5
Y1 - 1989/5
N2 - The goal of this study was to delineate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP‐specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78%) than in normal donors (31%), and were an important prerequisite for the successful selection of MBP‐specific T cell lines.
AB - The goal of this study was to delineate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP‐specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78%) than in normal donors (31%), and were an important prerequisite for the successful selection of MBP‐specific T cell lines.
KW - MBP‐specific T lymphocyte lines
KW - T cell proliferation
KW - electrophoretic analysis of MBP
KW - experimental autoimmune encephalomyelitis (EAE)
KW - human CNS diseases
KW - multiple sclerosis
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U2 - 10.1002/jnr.490230104
DO - 10.1002/jnr.490230104
M3 - Article
C2 - 2473214
AN - SCOPUS:0024337051
SN - 0360-4012
VL - 23
SP - 21
EP - 30
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 1
ER -