Human T lymphocyte response to myelin basic protein

Selection of T lymphocyte lines from MBP-responsive donors

Arthur Vandenbark, Y. K. Chou, Dennis Bourdette, Ruth Whitham, J. Chilgren, C. H J Chou, G. Konat, G. Hashim, M. Vainiene, Halina Offner

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The goal of this study was to delinate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP-specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78%) than in normal donors (31%), and were an important prerequisite for the successful selection of MBP-specific T cell lines.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalJournal of Neuroscience Research
Volume23
Issue number1
StatePublished - 1989

Fingerprint

Myelin Basic Protein
Tissue Donors
T-Lymphocytes
Multiple Sclerosis
Nervous System Diseases
Epitopes
Blood Cells
Cell Proliferation
Cell Line
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Human T lymphocyte response to myelin basic protein : Selection of T lymphocyte lines from MBP-responsive donors. / Vandenbark, Arthur; Chou, Y. K.; Bourdette, Dennis; Whitham, Ruth; Chilgren, J.; Chou, C. H J; Konat, G.; Hashim, G.; Vainiene, M.; Offner, Halina.

In: Journal of Neuroscience Research, Vol. 23, No. 1, 1989, p. 21-30.

Research output: Contribution to journalArticle

@article{f7b461366eee48bbb571b7facecb03af,
title = "Human T lymphocyte response to myelin basic protein: Selection of T lymphocyte lines from MBP-responsive donors",
abstract = "The goal of this study was to delinate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP-specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78{\%}) than in normal donors (31{\%}), and were an important prerequisite for the successful selection of MBP-specific T cell lines.",
author = "Arthur Vandenbark and Chou, {Y. K.} and Dennis Bourdette and Ruth Whitham and J. Chilgren and Chou, {C. H J} and G. Konat and G. Hashim and M. Vainiene and Halina Offner",
year = "1989",
language = "English (US)",
volume = "23",
pages = "21--30",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Human T lymphocyte response to myelin basic protein

T2 - Selection of T lymphocyte lines from MBP-responsive donors

AU - Vandenbark, Arthur

AU - Chou, Y. K.

AU - Bourdette, Dennis

AU - Whitham, Ruth

AU - Chilgren, J.

AU - Chou, C. H J

AU - Konat, G.

AU - Hashim, G.

AU - Vainiene, M.

AU - Offner, Halina

PY - 1989

Y1 - 1989

N2 - The goal of this study was to delinate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP-specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78%) than in normal donors (31%), and were an important prerequisite for the successful selection of MBP-specific T cell lines.

AB - The goal of this study was to delinate the importance of blood T lymphocyte responses to several myelin basic protein (MBP) preparations in the ultimate selection of MBP-specific T lymphocyte lines. Proliferation responses to human myelin basic protein (MBP) were assessed in blood samples from 27 multiple sclerosis (MS) patients, 20 patients with other neurologic diseases (OND), and 26 normal subjects, using five MBP preparations with different histories and electrophoretic characteristics to enhance the spectrum of epitopes represented. Substantial variations were observed in the ability of different MBP preparations to induce blood T cell proliferation in a given donor. However, four out of five of the MBPs induced modest but significant proliferation in the MS study population relative to normal individuals, with intermediate responses occurring in OND patients. Positive responses occurred more frequently in MS patients (78%) than in normal donors (31%), and were an important prerequisite for the successful selection of MBP-specific T cell lines.

UR - http://www.scopus.com/inward/record.url?scp=0024337051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024337051&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 21

EP - 30

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 1

ER -