Human proto-oncogene c-kit

a new cell surface receptor tyrosine kinase for an unidentified ligand.

Y. Yarden, W. J. Kuang, T. Yang-Feng, Lisa Coussens, S. Munemitsu, T. J. Dull, E. Chen, J. Schlessinger, U. Francke, A. Ullrich

Research output: Contribution to journalArticle

1229 Citations (Scopus)

Abstract

Structural features of v-kit, the oncogene of HZ4 feline sarcoma virus, suggested that this gene arose by transduction and truncation of cellular sequences. Complementary DNA cloning of the human proto-oncogene coding for a receptor tyrosine kinase confirmed this possibility: c-kit encodes a transmembrane glycoprotein that is structurally related to the receptor for macrophage growth factor (CSF-1) and the receptor for platelet-derived growth factor. The c-kit gene is widely expressed as a single, 5-kb transcript, and it is localized to human chromosome 4 and to mouse chromosome 5. A c-kit peptide antibody permitted the identification of a 145,000 dalton c-kit gene product that is inserted in the cellular plasma membrane and is capable of self-phosphorylation on tyrosine residues in both human glioblastoma cells and transfected mouse fibroblasts. Our results suggest that p145c-kit functions as a cell surface receptor for an as yet unidentified ligand. Furthermore, carboxy- and amino-terminal truncations that occurred during the viral transduction process are likely to have generated the transformation potential of v-kit.

Original languageEnglish (US)
Pages (from-to)3341-3351
Number of pages11
JournalEMBO Journal
Volume6
Issue number11
StatePublished - Nov 1987
Externally publishedYes

Fingerprint

Proto-Oncogenes
Cell Surface Receptors
Protein-Tyrosine Kinases
Genes
Chromosomes
Ligands
Feline Sarcoma Viruses
Macrophage Colony-Stimulating Factor Receptors
Proto-Oncogene Proteins c-kit
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 4
Phosphorylation
Growth Factor Receptors
Macrophages
Cloning
Platelet-Derived Growth Factor
Receptor Protein-Tyrosine Kinases
Human Chromosomes
Fibroblasts
Cell membranes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Yarden, Y., Kuang, W. J., Yang-Feng, T., Coussens, L., Munemitsu, S., Dull, T. J., ... Ullrich, A. (1987). Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO Journal, 6(11), 3341-3351.

Human proto-oncogene c-kit : a new cell surface receptor tyrosine kinase for an unidentified ligand. / Yarden, Y.; Kuang, W. J.; Yang-Feng, T.; Coussens, Lisa; Munemitsu, S.; Dull, T. J.; Chen, E.; Schlessinger, J.; Francke, U.; Ullrich, A.

In: EMBO Journal, Vol. 6, No. 11, 11.1987, p. 3341-3351.

Research output: Contribution to journalArticle

Yarden, Y, Kuang, WJ, Yang-Feng, T, Coussens, L, Munemitsu, S, Dull, TJ, Chen, E, Schlessinger, J, Francke, U & Ullrich, A 1987, 'Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.', EMBO Journal, vol. 6, no. 11, pp. 3341-3351.
Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ et al. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO Journal. 1987 Nov;6(11):3341-3351.
Yarden, Y. ; Kuang, W. J. ; Yang-Feng, T. ; Coussens, Lisa ; Munemitsu, S. ; Dull, T. J. ; Chen, E. ; Schlessinger, J. ; Francke, U. ; Ullrich, A. / Human proto-oncogene c-kit : a new cell surface receptor tyrosine kinase for an unidentified ligand. In: EMBO Journal. 1987 ; Vol. 6, No. 11. pp. 3341-3351.
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