Human platelet glycoprotein V: Its role in enhancing expression of the glycoprotein Ib receptor

D. C. Calverley, M. Yagi, S. M. Stray, G. J. Roth

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    Abstract

    Platelet adhesion to an injured blood vessel well is a critical initiating step in hemostasis mediated by a four member receptor complex (glycoprotein Ib/V/IX) interacting with plasma von Willebrand factor (vWF). The function of the GPV subunit within this complex is presently undefined. To study the role of glycoprotein (GP) V within the GPIb receptor complex, we transfected the GPV subunit gene into a hematopoietic cell line that constitutively expresses the other three subunits (human erythroleukemia [HEL] cells). Using flow cytometry, we found transfected GPV was surface expressed in HEL cells; this, in turn, led to increased surface expression of the ligand-binding GPIbα and GPIX subunits. Radioligand binding assays showed that GPV-transfected HEL cells bound more vWF than their non- or mock-transfected counterparts. We employed confocal microscopy of GPV-transfected HEL cells to show that GPV colocalizes with GPIbα on the cell surface. These findings suggest that the GPV subunit plays a role within the GPIb receptor complex by enhancing Ibα surface expression.

    Original languageEnglish (US)
    Pages (from-to)1361-1367
    Number of pages7
    JournalBlood
    Volume86
    Issue number4
    StatePublished - Jan 1 1995

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    ASJC Scopus subject areas

    • Biochemistry
    • Immunology
    • Hematology
    • Cell Biology

    Cite this

    Calverley, D. C., Yagi, M., Stray, S. M., & Roth, G. J. (1995). Human platelet glycoprotein V: Its role in enhancing expression of the glycoprotein Ib receptor. Blood, 86(4), 1361-1367.