Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2

Afam Okoye, P. Cordano, E. R. Taylor, I. M. Morgan, R. Everett, M. S. Campo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study we analysed the outcome of the interaction between HPV-16 L2 and E2 on the transactivation and DNA replication functions of E2. When E2 was expressed on its own, it transactivated a number of E2-responsive promoters but co-expression of L2 led to the down-regulation of the transcription transactivation activity of the E2 protein. This repression is not mediated by an increased degradation of the E2 protein. In contrast, the expression of L2 had no effect on the ability of E2 to activate DNA replication in association with the viral replication factor E1. Deletion mutagenesis identified L2 domains responsible for binding to E2 (first 50 N-terminus amino acid residues) and down-regulating its transactivation function (residues 301-400). The results demonstrate that L2 selectively inhibits the transcriptional activation property of E2 and that there is a direct interaction between the two proteins, although this is not sufficient to mediate the transcriptional repression. The consequences of the L2-E2 interaction for the viral life cycle are discussed.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalVirus Research
Volume108
Issue number1-2
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Human papillomavirus 16
DNA Replication
Transcriptional Activation
Life Cycle Stages
Mutagenesis
Proteolysis
Proteins
Down-Regulation
Outcome Assessment (Health Care)
Amino Acids

Keywords

  • E2
  • HPV-16
  • L2
  • Transcription regulation
  • Viral DNA replication

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2. / Okoye, Afam; Cordano, P.; Taylor, E. R.; Morgan, I. M.; Everett, R.; Campo, M. S.

In: Virus Research, Vol. 108, No. 1-2, 03.2005, p. 1-14.

Research output: Contribution to journalArticle

Okoye, Afam ; Cordano, P. ; Taylor, E. R. ; Morgan, I. M. ; Everett, R. ; Campo, M. S. / Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2. In: Virus Research. 2005 ; Vol. 108, No. 1-2. pp. 1-14.
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