Human myelin basic protein (MBP) epitopes recognized by mouse MBP-selected T cell lines from multiple sclerosis patients

Yuan K. Chou, Richard E. Jones, Dennis Bourdette, Ruth Whithman, George Hashim, Jeanette Atherton, Halina Offner, Arthur A. Vandenbark

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We investigated whether myelin basic protein (MBP)-reactive T cells from multiple sclerosis (MS) patients can recognize mouse MBP since this is an expected requirement for the transfer of experimental autoimmune encephalomyelitis (EAE) into severe combined immunodeficiency (SCID) mouse-human chimeras. Peripheral blood mononuclear cells from 11 MS patients were analyzed for in vitro proliferation to mouse MBP. Six patients (55%) responded to mouse MBP at the first or second stimulation. Five T cell lines, selected with mouse MBP from five MS patients, were analyzed for their proliferation to mouse and human MBP and to a panel of synthetic peptides of human MBP. Four of the five lines recognized mouse MBP. In vitro proliferation was restricted by MHC class II in one line tested for MHC restriction. One of the five lines recognized whole human MBP and all five of the lines responded to at least one of the five synthetic peptides corresponding to human MBP residues 8-28, 67-90, 84-102, 87-99 or 130-149. These results show that MS patient T cells recognize mouse MBP and suggest that distinct human MBP epitopes are immunologically cross-reactive with epitopes of mouse MBP.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalJournal of Neuroimmunology
Volume49
Issue number1-2
DOIs
StatePublished - Jan 1994

Keywords

  • Epitope
  • Experimental autoimmune encephalomyelitis
  • Human
  • Mouse
  • Multiple sclerosis
  • Myelin basic protein
  • SCID mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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