Human monocyte activation by biologic and biodegradable meshes in vitro

Sean Orenstein, Yi Qiao, Manjot Kaur, Ulrike Klueh, Don L. Kreutzer, Yuri W. Novitsky

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background: Inflammation and wound healing play critical roles in the integration of biologic and biodegradable meshes (BMs) at hernia repair sites. Monocytes/macrophages (M/MØs) are key cells controlling inflammation and wound healing. These cells release inflammatory cytokines and growth factors such as interleukin (IL)-1b, IL-6, IL-8, and vascular endothelial growth factor (VEGF) upon activation. Although BMs have been increasingly used in hernia repairs worldwide, to date, investigations of inflammatory responses to various BMs have been limited. Methods Mesh samples of three acellular human dermisderived biologic meshes (AlloDerm, AlloMax, FlexHD) and one biodegradable synthetic mesh (Bio-A) were placed in 96-well plates. Human peripheral blood mononuclear cells (PBMCs) were isolated from six healthy subjects, added to each well, and incubated for 7 days. Culture supernatants were assayed for IL-1b, IL-6, IL-8, and VEGF levels using a multiplex bead-base immunoassay system (Bio-Plex). Results All four meshes induced cytokine expression from activated M/MØs to varying degrees in vitro. FlexHD induced significantly more IL-1b (2,591 pg/ml) than AlloMax (517 pg/ml), AlloDerm (48 pg/ml), or Bio-A (28 pg/ml) (p\0.001). AlloMax stimulated a significantly greater quantity of IL-6 (38,343 pg/ml) than FlexHD (19,317 pg/ml), Bio-A (191 pg/ml), or AlloDerm (103 pg/ ml) (p\0.05). Interleukin-8 and VEGF displayed trends similar to that of IL-6. There were no significant differences in cytokine production between AlloDerm and Bio-A. Conclusion This study demonstrated that human macrophages are activated by human dermis-derived biologic and biodegradable meshes in vitro. A wide range of cytokine and growth factor induction was seen among the different mesh products. These differences in M/MØ activation may be related to the proprietary processing technologies of the studied meshes. The study results raise the possibility that these differences in M/MØ activation could indicate varying intensities of inflammation that control integration of different biologic meshes at the sites of hernia repair.

Original languageEnglish (US)
Pages (from-to)805-811
Number of pages7
JournalSurgical Endoscopy and Other Interventional Techniques
Volume24
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

Fingerprint

Monocytes
Herniorrhaphy
Interleukin-6
Interleukin-8
Vascular Endothelial Growth Factor A
Cytokines
Interleukins
Macrophages
Inflammation
Wound Healing
Intercellular Signaling Peptides and Proteins
Dermis
Immunoassay
Interleukin-2
Blood Cells
Healthy Volunteers
Technology
Alloderm
In Vitro Techniques
FlexHD

Keywords

  • Biologic mesh
  • Cytokine
  • Growth factor
  • Human dermis
  • Monocyte/macrophage
  • Mononuclear cells

ASJC Scopus subject areas

  • Surgery

Cite this

Human monocyte activation by biologic and biodegradable meshes in vitro. / Orenstein, Sean; Qiao, Yi; Kaur, Manjot; Klueh, Ulrike; Kreutzer, Don L.; Novitsky, Yuri W.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 24, No. 4, 04.2010, p. 805-811.

Research output: Contribution to journalArticle

Orenstein, Sean ; Qiao, Yi ; Kaur, Manjot ; Klueh, Ulrike ; Kreutzer, Don L. ; Novitsky, Yuri W. / Human monocyte activation by biologic and biodegradable meshes in vitro. In: Surgical Endoscopy and Other Interventional Techniques. 2010 ; Vol. 24, No. 4. pp. 805-811.
@article{c6c8c3f83a184c48acb474a71046d908,
title = "Human monocyte activation by biologic and biodegradable meshes in vitro",
abstract = "Background: Inflammation and wound healing play critical roles in the integration of biologic and biodegradable meshes (BMs) at hernia repair sites. Monocytes/macrophages (M/M{\O}s) are key cells controlling inflammation and wound healing. These cells release inflammatory cytokines and growth factors such as interleukin (IL)-1b, IL-6, IL-8, and vascular endothelial growth factor (VEGF) upon activation. Although BMs have been increasingly used in hernia repairs worldwide, to date, investigations of inflammatory responses to various BMs have been limited. Methods Mesh samples of three acellular human dermisderived biologic meshes (AlloDerm, AlloMax, FlexHD) and one biodegradable synthetic mesh (Bio-A) were placed in 96-well plates. Human peripheral blood mononuclear cells (PBMCs) were isolated from six healthy subjects, added to each well, and incubated for 7 days. Culture supernatants were assayed for IL-1b, IL-6, IL-8, and VEGF levels using a multiplex bead-base immunoassay system (Bio-Plex). Results All four meshes induced cytokine expression from activated M/M{\O}s to varying degrees in vitro. FlexHD induced significantly more IL-1b (2,591 pg/ml) than AlloMax (517 pg/ml), AlloDerm (48 pg/ml), or Bio-A (28 pg/ml) (p\0.001). AlloMax stimulated a significantly greater quantity of IL-6 (38,343 pg/ml) than FlexHD (19,317 pg/ml), Bio-A (191 pg/ml), or AlloDerm (103 pg/ ml) (p\0.05). Interleukin-8 and VEGF displayed trends similar to that of IL-6. There were no significant differences in cytokine production between AlloDerm and Bio-A. Conclusion This study demonstrated that human macrophages are activated by human dermis-derived biologic and biodegradable meshes in vitro. A wide range of cytokine and growth factor induction was seen among the different mesh products. These differences in M/M{\O} activation may be related to the proprietary processing technologies of the studied meshes. The study results raise the possibility that these differences in M/M{\O} activation could indicate varying intensities of inflammation that control integration of different biologic meshes at the sites of hernia repair.",
keywords = "Biologic mesh, Cytokine, Growth factor, Human dermis, Monocyte/macrophage, Mononuclear cells",
author = "Sean Orenstein and Yi Qiao and Manjot Kaur and Ulrike Klueh and Kreutzer, {Don L.} and Novitsky, {Yuri W.}",
year = "2010",
month = "4",
doi = "10.1007/s00464-009-0664-3",
language = "English (US)",
volume = "24",
pages = "805--811",
journal = "Surgical Endoscopy and Other Interventional Techniques",
issn = "0930-2794",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Human monocyte activation by biologic and biodegradable meshes in vitro

AU - Orenstein, Sean

AU - Qiao, Yi

AU - Kaur, Manjot

AU - Klueh, Ulrike

AU - Kreutzer, Don L.

AU - Novitsky, Yuri W.

PY - 2010/4

Y1 - 2010/4

N2 - Background: Inflammation and wound healing play critical roles in the integration of biologic and biodegradable meshes (BMs) at hernia repair sites. Monocytes/macrophages (M/MØs) are key cells controlling inflammation and wound healing. These cells release inflammatory cytokines and growth factors such as interleukin (IL)-1b, IL-6, IL-8, and vascular endothelial growth factor (VEGF) upon activation. Although BMs have been increasingly used in hernia repairs worldwide, to date, investigations of inflammatory responses to various BMs have been limited. Methods Mesh samples of three acellular human dermisderived biologic meshes (AlloDerm, AlloMax, FlexHD) and one biodegradable synthetic mesh (Bio-A) were placed in 96-well plates. Human peripheral blood mononuclear cells (PBMCs) were isolated from six healthy subjects, added to each well, and incubated for 7 days. Culture supernatants were assayed for IL-1b, IL-6, IL-8, and VEGF levels using a multiplex bead-base immunoassay system (Bio-Plex). Results All four meshes induced cytokine expression from activated M/MØs to varying degrees in vitro. FlexHD induced significantly more IL-1b (2,591 pg/ml) than AlloMax (517 pg/ml), AlloDerm (48 pg/ml), or Bio-A (28 pg/ml) (p\0.001). AlloMax stimulated a significantly greater quantity of IL-6 (38,343 pg/ml) than FlexHD (19,317 pg/ml), Bio-A (191 pg/ml), or AlloDerm (103 pg/ ml) (p\0.05). Interleukin-8 and VEGF displayed trends similar to that of IL-6. There were no significant differences in cytokine production between AlloDerm and Bio-A. Conclusion This study demonstrated that human macrophages are activated by human dermis-derived biologic and biodegradable meshes in vitro. A wide range of cytokine and growth factor induction was seen among the different mesh products. These differences in M/MØ activation may be related to the proprietary processing technologies of the studied meshes. The study results raise the possibility that these differences in M/MØ activation could indicate varying intensities of inflammation that control integration of different biologic meshes at the sites of hernia repair.

AB - Background: Inflammation and wound healing play critical roles in the integration of biologic and biodegradable meshes (BMs) at hernia repair sites. Monocytes/macrophages (M/MØs) are key cells controlling inflammation and wound healing. These cells release inflammatory cytokines and growth factors such as interleukin (IL)-1b, IL-6, IL-8, and vascular endothelial growth factor (VEGF) upon activation. Although BMs have been increasingly used in hernia repairs worldwide, to date, investigations of inflammatory responses to various BMs have been limited. Methods Mesh samples of three acellular human dermisderived biologic meshes (AlloDerm, AlloMax, FlexHD) and one biodegradable synthetic mesh (Bio-A) were placed in 96-well plates. Human peripheral blood mononuclear cells (PBMCs) were isolated from six healthy subjects, added to each well, and incubated for 7 days. Culture supernatants were assayed for IL-1b, IL-6, IL-8, and VEGF levels using a multiplex bead-base immunoassay system (Bio-Plex). Results All four meshes induced cytokine expression from activated M/MØs to varying degrees in vitro. FlexHD induced significantly more IL-1b (2,591 pg/ml) than AlloMax (517 pg/ml), AlloDerm (48 pg/ml), or Bio-A (28 pg/ml) (p\0.001). AlloMax stimulated a significantly greater quantity of IL-6 (38,343 pg/ml) than FlexHD (19,317 pg/ml), Bio-A (191 pg/ml), or AlloDerm (103 pg/ ml) (p\0.05). Interleukin-8 and VEGF displayed trends similar to that of IL-6. There were no significant differences in cytokine production between AlloDerm and Bio-A. Conclusion This study demonstrated that human macrophages are activated by human dermis-derived biologic and biodegradable meshes in vitro. A wide range of cytokine and growth factor induction was seen among the different mesh products. These differences in M/MØ activation may be related to the proprietary processing technologies of the studied meshes. The study results raise the possibility that these differences in M/MØ activation could indicate varying intensities of inflammation that control integration of different biologic meshes at the sites of hernia repair.

KW - Biologic mesh

KW - Cytokine

KW - Growth factor

KW - Human dermis

KW - Monocyte/macrophage

KW - Mononuclear cells

UR - http://www.scopus.com/inward/record.url?scp=77952549553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952549553&partnerID=8YFLogxK

U2 - 10.1007/s00464-009-0664-3

DO - 10.1007/s00464-009-0664-3

M3 - Article

C2 - 19697086

AN - SCOPUS:77952549553

VL - 24

SP - 805

EP - 811

JO - Surgical Endoscopy and Other Interventional Techniques

JF - Surgical Endoscopy and Other Interventional Techniques

SN - 0930-2794

IS - 4

ER -