A direct quantitative and phenotypic cytofluorographic analysis of TCR-γ/δ+ lymphocytes as well as an immunohistologic study of their tissue distribution and microanatomy was made possible by the availability of two mAbs (anti-TCR-δ1 and anti-CγM1) specific for framework determinants on human TCR γ and δ chains, respectively. TCR-γ/δ+ lymphocytes, ranging between >0.5 and 16% of CD3+ cells, were found in fetal and postnatal thymus, fetal and adult peripheral lymphoid organs, and adult peripheral blood. While TCR-γ/δ+ lymphocytes comprised a small subpopulation of T cells (mean, ~4%) occasionally >10-16% of CD3+ cells expressed TCR-γ/δ. Virtually all TCR-γ/δ+ thymocytes/lymphocytes expressed CD7, CD2, and CD5 but were heterogeneous with respect to their expression of CD1, CD4, CD8, CD28, CD11b, CD16, and Leu-7. Human TCR-γ/δ+ cells populate both organized lymphoid tissues (thymus, tonsil, lymphnode, and spleen) as well as the gut- and skin-associated lymphoid systems at similar frequencies without obvious tropism for epithelial microenvironments. TCR-γ/δ+ lymphocytes tend to be located within a given organ wherever TCR-α/β+ lymphocytes are found. This study shows that TCR-γ/δ+ lymphocytes constitute a small but numerically important, phenotypically diverse T cell population distributed throughout the body. These results support the concept that TCR-γ/δ+ cells comprise a distinct, functionally heterogeneous, mature T cell sublineage that may substantially broaden the T cell repertoire at all immunologically relevant sites.
|Original language||English (US)|
|Number of pages||18|
|Journal||Journal of Experimental Medicine|
|Publication status||Published - 1989|
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