Human insulinlike growth factor 1 gene transfer into paralyzed rat larynx: Single vs multiple injection

Akihiro Shiotani, Bert W. O'Malley, Michael E. Coleman, Paul Flint

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective: To compare the biological effects of single vs multiple treatment of rat denervated laryngeal muscle with human insulinlike growth factor 1 (hIGF1) gene therapy. Experimental Methods or Design: A muscle- specific nonviral vector containing the α-actin promoter and hIGF1 gene formulated with polyvinyl polymers was injected into denervated adult rat thyroarytenoid muscle. The effects on animals given a single injection (n = 16) vs those given multiple injections (n = 14) vs control groups (n = 18) were evaluated. Twenty-eight days after the first injection, gene expression, muscle fiber size, motor endplate length, and nerve-to-motor endplate contact were evaluated. Results: Gene expression, detected by reverse transcriptase polymerase chain reaction for hIGF1 messenger RNA, occurred in 13 (81%) of 16 animals receiving single injections and 14 (100%) of 14 animals receiving multiple injections. Compared with controls, hIGF1-transfected animals in both single- and multiple- injection groups had a significant increase in the lesser diameter of muscle fiber, a significant decrease in motor endplate length, and a significant increase in the percentage of endplates with nerve contact (P

Original languageEnglish (US)
Pages (from-to)555-560
Number of pages6
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume125
Issue number5
StatePublished - May 1999
Externally publishedYes

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Larynx
Intercellular Signaling Peptides and Proteins
Motor Endplate
Injections
Genes
Laryngeal Muscles
Muscles
Polyvinyls
Gene Expression
Reverse Transcriptase Polymerase Chain Reaction
Genetic Therapy
Actins
Polymers
Control Groups
Messenger RNA

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Human insulinlike growth factor 1 gene transfer into paralyzed rat larynx : Single vs multiple injection. / Shiotani, Akihiro; O'Malley, Bert W.; Coleman, Michael E.; Flint, Paul.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 125, No. 5, 05.1999, p. 555-560.

Research output: Contribution to journalArticle

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