Human immunodeficiency virus type 1 matrix protein assembles on membranes as a hexamer

Ayna Alfadhli, Doug Huseby, Eliot Kapit, Dalbinder Colman, Eric Barklis

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The membrane-binding matrix (MA) domain of the human immunodeficiency virus type 1 (HIV-1) structural precursor Gag (PrGag) protein oligomerizes in solution as a trimer and crystallizes in three dimensions as a trimer unit. A number of models have been proposed to explain how MA trimers might align with respect to PrGag capsid (CA) N-terminal domains (NTDs), which assemble hexagonal lattices. We have examined the binding of naturally myristoylated HIV-1 matrix (MyrMA) and matrix plus capsid (MyrMACA) proteins on membranes in vitro. Unexpectedly, MyrMA and MyrMACA proteins both assembled hexagonal cage lattices on phosphatidylserine-cholesterol membranes. Membrane-bound MyrMA proteins did not organize into trimer units but, rather, organized into hexamer rings. Our results yield a model in which MA domains stack directly above NTD hexamers in immature particles, and they have implications for IUV assembly and interactions between MA and the viral membrane glycoproteins.

Original languageEnglish (US)
Pages (from-to)1472-1478
Number of pages7
JournalJournal of Virology
Volume81
Issue number3
DOIs
StatePublished - Feb 2007

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Human immunodeficiency virus 1
HIV-1
Membranes
Capsid Proteins
coat proteins
Proteins
proteins
membrane glycoproteins
gag Gene Products
phosphatidylserines
capsid
Capsid
Phosphatidylserines
Membrane Glycoproteins
cages
immatures
Cholesterol
cholesterol

ASJC Scopus subject areas

  • Immunology

Cite this

Human immunodeficiency virus type 1 matrix protein assembles on membranes as a hexamer. / Alfadhli, Ayna; Huseby, Doug; Kapit, Eliot; Colman, Dalbinder; Barklis, Eric.

In: Journal of Virology, Vol. 81, No. 3, 02.2007, p. 1472-1478.

Research output: Contribution to journalArticle

Alfadhli, Ayna ; Huseby, Doug ; Kapit, Eliot ; Colman, Dalbinder ; Barklis, Eric. / Human immunodeficiency virus type 1 matrix protein assembles on membranes as a hexamer. In: Journal of Virology. 2007 ; Vol. 81, No. 3. pp. 1472-1478.
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