Human immunodeficiency virus infection of human brain capillary endothelial cells occurs via a CD4/galactosylceramide-independent mechanism

Ashlee Moses, Floyd E. Bloom, C. David Pauza, Jay Nelson

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

Neuropathologic studies of AIDS patients have shown that brain capillary endothelial cells are a cellular target for human immunodeficiency virus (HIV) in vivo. We have established in vitro cultures of primary human brain capillary endothelial (HBCE) cells. Using this model system, we have shown a significant HIV infection of HBCE cells that is productive yet noncytopathic. The infection is mediated by a cellular interaction with gp120 that does not involve CD4 or galactosylceramide. HIV infection of HBCE cells may contribute to AIDS-associated neuropathology by disturbing the physiology of the endothelium and directly or indirectly facilitating dissemination of virus to the central nervous system.

Original languageEnglish (US)
Pages (from-to)10474-10478
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number22
StatePublished - Nov 15 1993

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Galactosylceramides
Virus Diseases
Endothelial Cells
HIV
Brain
Acquired Immunodeficiency Syndrome
Endothelium
Central Nervous System
Viruses
Infection

Keywords

  • AIDS
  • Blood-brain barrier
  • Neurologic disease

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AU - Moses, Ashlee

AU - Bloom, Floyd E.

AU - Pauza, C. David

AU - Nelson, Jay

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AB - Neuropathologic studies of AIDS patients have shown that brain capillary endothelial cells are a cellular target for human immunodeficiency virus (HIV) in vivo. We have established in vitro cultures of primary human brain capillary endothelial (HBCE) cells. Using this model system, we have shown a significant HIV infection of HBCE cells that is productive yet noncytopathic. The infection is mediated by a cellular interaction with gp120 that does not involve CD4 or galactosylceramide. HIV infection of HBCE cells may contribute to AIDS-associated neuropathology by disturbing the physiology of the endothelium and directly or indirectly facilitating dissemination of virus to the central nervous system.

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