Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells

William Fleming, P. R. Murphy, L. J. Murphy, T. W. Hatton, R. J. Matusik, H. G. Friesen

Research output: Contribution to journalArticle

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Abstract

The effect of human GH (hGH) on c-myc gene expression and cell proliferation in the Nb2 lymphoma cell line was examined. The addition of hormone to stationary cultures of Nb2 cells resulted in an increased accumulation of c-myc transcripts that was detectable within 15 min, reached a maximum induction of 25-fold in 3 h, and then gradually declined. Although maximal accumulation of c-myc transcripts occurred within 3 h after hGH addition, removal of the hormone even after 4 h of treatment resulted in a failure of the cells to proliferate. This loss of proliferative capacity following hormone removal was accompanied by a 20-fold reduction in the level of c-myc transcripts. These results indicate that hGH induced proliferation of Nb2 cells is associated with the induction and maintenance of c-myc gene expression.

Original languageEnglish (US)
Pages (from-to)2547-2549
Number of pages3
JournalEndocrinology
Volume117
Issue number6
StatePublished - 1985
Externally publishedYes

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myc Genes
Human Growth Hormone
Lymphoma
Hormones
Gene Expression
Cell Proliferation
Cell Culture Techniques
Maintenance
Cell Line

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Fleming, W., Murphy, P. R., Murphy, L. J., Hatton, T. W., Matusik, R. J., & Friesen, H. G. (1985). Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells. Endocrinology, 117(6), 2547-2549.

Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells. / Fleming, William; Murphy, P. R.; Murphy, L. J.; Hatton, T. W.; Matusik, R. J.; Friesen, H. G.

In: Endocrinology, Vol. 117, No. 6, 1985, p. 2547-2549.

Research output: Contribution to journalArticle

Fleming, W, Murphy, PR, Murphy, LJ, Hatton, TW, Matusik, RJ & Friesen, HG 1985, 'Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells', Endocrinology, vol. 117, no. 6, pp. 2547-2549.
Fleming W, Murphy PR, Murphy LJ, Hatton TW, Matusik RJ, Friesen HG. Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells. Endocrinology. 1985;117(6):2547-2549.
Fleming, William ; Murphy, P. R. ; Murphy, L. J. ; Hatton, T. W. ; Matusik, R. J. ; Friesen, H. G. / Human growth hormone induces and maintains C-myc gene expression in Nb2 lymphoma cells. In: Endocrinology. 1985 ; Vol. 117, No. 6. pp. 2547-2549.
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