Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids

Mary R. Bordelon, H. G. Coon, P. O. Kohler

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.

Original languageEnglish (US)
Pages (from-to)303-310
Number of pages8
JournalExperimental Cell Research
Volume103
Issue number2
DOIs
StatePublished - Dec 1976

ASJC Scopus subject areas

  • Cell Biology

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