Human Genes Encoding the Voltage-Dependent Anion Channel (VDAC) of the Outer Mitochondrial Membrane

Mapping and Identification of Two New Isoforms

Elizabeth Blachly-Dyson, Antonio Baldini, Michael Litt, Edward R B McCabe, Michael Forte

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The voltage-dependent anion channel of the mitochondrial outer membrane (VDAC) is a small, abundant pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is believed to form the major path way for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. Previous studies have indicated that at least two human VDAC isoforms are expressed. Here, we report the mapping of VDAC1 to the X chromosome in the interval Xq13-q21 and VDAC2 to chromosome 21 by polymerase chain reaction and restriction analysis of a human/rodent somatic cell mapping panel. In the process of mapping these genes, we identified and mapped two additional sequences highly homologous to VDAC1. VDAC3 maps to chromosome 12 and VDAC4 maps to chromosome 1. The locations of VDAC1 and VDAC4 have been confirmed by fluorescence in situ hybridization analysis. Future studies will be aimed at defining the specific physiological role of each member of this family of channel proteins.

Original languageEnglish (US)
Pages (from-to)62-67
Number of pages6
JournalGenomics
Volume20
Issue number1
DOIs
StatePublished - Mar 1 1994

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Voltage-Dependent Anion Channels
Mitochondrial Membranes
Protein Isoforms
Genes
Glycerol Kinase
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 21
Porins
Hexokinase
Membranes
Adenine Nucleotides
Chromosome Mapping
Chromosomes, Human, Pair 1
X Chromosome
Sequence Homology
Fluorescence In Situ Hybridization
Rodentia
Mitochondria
Proteins
Binding Sites

ASJC Scopus subject areas

  • Genetics

Cite this

Human Genes Encoding the Voltage-Dependent Anion Channel (VDAC) of the Outer Mitochondrial Membrane : Mapping and Identification of Two New Isoforms. / Blachly-Dyson, Elizabeth; Baldini, Antonio; Litt, Michael; McCabe, Edward R B; Forte, Michael.

In: Genomics, Vol. 20, No. 1, 01.03.1994, p. 62-67.

Research output: Contribution to journalArticle

Blachly-Dyson, Elizabeth ; Baldini, Antonio ; Litt, Michael ; McCabe, Edward R B ; Forte, Michael. / Human Genes Encoding the Voltage-Dependent Anion Channel (VDAC) of the Outer Mitochondrial Membrane : Mapping and Identification of Two New Isoforms. In: Genomics. 1994 ; Vol. 20, No. 1. pp. 62-67.
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