Human gastrin-releasing peptide gene is located on chromosome 18

S. L. Naylor, A. Y. Sakaguchi, Eliot Spindel, W. W. Chin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Gastrin-releasing peptide (GRP), a bombesin-like peptide, increases plasma levels of gastrin, pancreatic polypeptide, glucagon, gastric inhibitory peptide, and insulin. GRP is produced in large quantities by small-cell lung cancer and acts as a growth factor for these cells. To determine if chromosomal changes in small-cell lung cancer are related to the expression of GRP, we chromosomally mapped the gene using human-mouse somatic cell hybrids. Twenty hybrids, characterized for human chromosomes, were analyzed by Southern filter hybridization of DNA digested with EcoRI. Human DNA cut with EcoRI yields a major band of 6.8 kb and a minor band of 11.3 kb. The 6.8 kb band segregated concordantly with chromosome 18 and the marker peptidase A. The chromosome 3 abnormalities seen in small-cell lung cancer do not correlate with the chromosomal location of GRP, suggesting that the elevated expression of this gene may be due to mechanisms other than chromosomal rearrangement.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalSomatic Cell and Molecular Genetics
Volume13
Issue number1
DOIs
StatePublished - Jan 1987
Externally publishedYes

Fingerprint

Gastrin-Releasing Peptide
Chromosomes, Human, Pair 18
Small Cell Lung Carcinoma
Genes
Pancreatic Polypeptide
Bombesin
Peptides
Chromosomes, Human, Pair 3
Hybrid Cells
DNA
Gastrins
Human Chromosomes
Glucagon
Genetic Markers
Chromosome Aberrations
Intercellular Signaling Peptides and Proteins
Stomach
Peptide Hydrolases
Insulin
Gene Expression

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Human gastrin-releasing peptide gene is located on chromosome 18. / Naylor, S. L.; Sakaguchi, A. Y.; Spindel, Eliot; Chin, W. W.

In: Somatic Cell and Molecular Genetics, Vol. 13, No. 1, 01.1987, p. 87-91.

Research output: Contribution to journalArticle

Naylor, S. L. ; Sakaguchi, A. Y. ; Spindel, Eliot ; Chin, W. W. / Human gastrin-releasing peptide gene is located on chromosome 18. In: Somatic Cell and Molecular Genetics. 1987 ; Vol. 13, No. 1. pp. 87-91.
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