Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

A. Ullrich, Lisa Coussens, J. S. Hayflick, T. J. Dull, A. Gray, A. W. Tam, J. Lee, Y. Yarden, T. A. Libermann, J. Schlessinger, J. Downward, E. L V Mayes, N. Whittle, M. D. Waterfield, P. H. Seeburg

Research output: Contribution to journalArticle

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Abstract

The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells, generating a truncated 2.8-kilobase mRNA which encodes only the extracellular EGF binding domain.

Original languageEnglish (US)
Pages (from-to)418-425
Number of pages8
JournalNature
Volume309
Issue number5967
DOIs
StatePublished - 1984
Externally publishedYes

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Epidermal Growth Factor Receptor
Epidermal Growth Factor
Squamous Cell Carcinoma
Complementary DNA
Gene Expression
Messenger RNA
Oncogene Proteins
Cytoplasmic and Nuclear Receptors
Amino Acid Sequence
Clone Cells
Carcinoma
Amino Acids
Genes

ASJC Scopus subject areas

  • General

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Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. / Ullrich, A.; Coussens, Lisa; Hayflick, J. S.; Dull, T. J.; Gray, A.; Tam, A. W.; Lee, J.; Yarden, Y.; Libermann, T. A.; Schlessinger, J.; Downward, J.; Mayes, E. L V; Whittle, N.; Waterfield, M. D.; Seeburg, P. H.

In: Nature, Vol. 309, No. 5967, 1984, p. 418-425.

Research output: Contribution to journalArticle

Ullrich, A, Coussens, L, Hayflick, JS, Dull, TJ, Gray, A, Tam, AW, Lee, J, Yarden, Y, Libermann, TA, Schlessinger, J, Downward, J, Mayes, ELV, Whittle, N, Waterfield, MD & Seeburg, PH 1984, 'Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells', Nature, vol. 309, no. 5967, pp. 418-425. https://doi.org/10.1038/309418a0
Ullrich, A. ; Coussens, Lisa ; Hayflick, J. S. ; Dull, T. J. ; Gray, A. ; Tam, A. W. ; Lee, J. ; Yarden, Y. ; Libermann, T. A. ; Schlessinger, J. ; Downward, J. ; Mayes, E. L V ; Whittle, N. ; Waterfield, M. D. ; Seeburg, P. H. / Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. In: Nature. 1984 ; Vol. 309, No. 5967. pp. 418-425.
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