Human endometriotic xenografts in immunodeficient RAG-2/γ(c)KO mice

Laura H. Greenberg, Ov Slayden

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Objective The purpose of this study was to create a novel animal model for studies of endometriosis. Study design To facilitate the study of the transplantation of endometriosis into immunodeficient RAG-2/γ(c)KO mice, endometriosis biopsy specimens were collected from 19 women by laparoscopic surgery and grafted subcutaneously into the mice, which were treated subsequently with estradiol and progesterone to create 28-day artificial cycles. The grafts were collected during the first, second, and fourth cycles and were evaluated histologically for evidence of bleeding and immunocytochemically for estrogen receptor and progesterone receptor. Results Biopsy specimens that contained endometrium-like glands were well accepted (>90% success). These grafts maintained glandular morphologic condition, estrogen receptor, and progesterone receptor; bled after progesterone withdrawal; and formed chocolate cysts. However, biopsy specimens that lacked glands or that consisted of peritoneal adhesions and stroma were accepted poorly

    Original languageEnglish (US)
    Pages (from-to)1788-1796
    Number of pages9
    JournalAmerican Journal of Obstetrics and Gynecology
    Volume190
    Issue number6
    DOIs
    StatePublished - Jun 2004

    Fingerprint

    Endometriosis
    Heterografts
    Progesterone Receptors
    Biopsy
    Estrogen Receptors
    Progesterone
    Transplants
    Endometrium
    Laparoscopy
    Cysts
    Estradiol
    Animal Models
    Transplantation
    Hemorrhage

    Keywords

    • Endometriosis
    • RAG-2/γ(c)KO mice
    • Xenograft

    ASJC Scopus subject areas

    • Medicine(all)
    • Obstetrics and Gynecology

    Cite this

    Human endometriotic xenografts in immunodeficient RAG-2/γ(c)KO mice. / Greenberg, Laura H.; Slayden, Ov.

    In: American Journal of Obstetrics and Gynecology, Vol. 190, No. 6, 06.2004, p. 1788-1796.

    Research output: Contribution to journalArticle

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    abstract = "Objective The purpose of this study was to create a novel animal model for studies of endometriosis. Study design To facilitate the study of the transplantation of endometriosis into immunodeficient RAG-2/γ(c)KO mice, endometriosis biopsy specimens were collected from 19 women by laparoscopic surgery and grafted subcutaneously into the mice, which were treated subsequently with estradiol and progesterone to create 28-day artificial cycles. The grafts were collected during the first, second, and fourth cycles and were evaluated histologically for evidence of bleeding and immunocytochemically for estrogen receptor and progesterone receptor. Results Biopsy specimens that contained endometrium-like glands were well accepted (>90{\%} success). These grafts maintained glandular morphologic condition, estrogen receptor, and progesterone receptor; bled after progesterone withdrawal; and formed chocolate cysts. However, biopsy specimens that lacked glands or that consisted of peritoneal adhesions and stroma were accepted poorly",
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    AB - Objective The purpose of this study was to create a novel animal model for studies of endometriosis. Study design To facilitate the study of the transplantation of endometriosis into immunodeficient RAG-2/γ(c)KO mice, endometriosis biopsy specimens were collected from 19 women by laparoscopic surgery and grafted subcutaneously into the mice, which were treated subsequently with estradiol and progesterone to create 28-day artificial cycles. The grafts were collected during the first, second, and fourth cycles and were evaluated histologically for evidence of bleeding and immunocytochemically for estrogen receptor and progesterone receptor. Results Biopsy specimens that contained endometrium-like glands were well accepted (>90% success). These grafts maintained glandular morphologic condition, estrogen receptor, and progesterone receptor; bled after progesterone withdrawal; and formed chocolate cysts. However, biopsy specimens that lacked glands or that consisted of peritoneal adhesions and stroma were accepted poorly

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