Human embryonic stem cells derived by somatic cell nuclear transfer

Masahito Tachibana, Paula Amato, Michelle Sparman, Nuria Marti Gutierrez, Rebecca Tippner-Hedges, Hong Ma, Eunju Kang, Alimujiang Fulati, Hyo Sang Lee, Hathaitip Sritanaudomchai, Keith Masterson, Janine Larson, Deborah Eaton, Karen Sadler-Fredd, David Battaglia, David Lee, Diana Wu, Jeffrey Jensen, Phillip Patton, Sumita Gokhale & 3 others Richard Stouffer, Don Wolf, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

426 Citations (Scopus)

Abstract

Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. PaperClip

Original languageEnglish (US)
Pages (from-to)1228-1238
Number of pages11
JournalCell
Volume153
Issue number6
DOIs
StatePublished - Jun 6 2013

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Embryonic Stem Cells
Stem cells
Oocytes
Pluripotent Stem Cells
Human Embryonic Stem Cells
Embryo Transfer
Meiosis
Diploidy
Karyotype
Transcriptome
Gene expression
Embryonic Structures
Genes
Chemical activation
Genome
Cell Line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tachibana, M., Amato, P., Sparman, M., Gutierrez, N. M., Tippner-Hedges, R., Ma, H., ... Mitalipov, S. (2013). Human embryonic stem cells derived by somatic cell nuclear transfer. Cell, 153(6), 1228-1238. https://doi.org/10.1016/j.cell.2013.05.006

Human embryonic stem cells derived by somatic cell nuclear transfer. / Tachibana, Masahito; Amato, Paula; Sparman, Michelle; Gutierrez, Nuria Marti; Tippner-Hedges, Rebecca; Ma, Hong; Kang, Eunju; Fulati, Alimujiang; Lee, Hyo Sang; Sritanaudomchai, Hathaitip; Masterson, Keith; Larson, Janine; Eaton, Deborah; Sadler-Fredd, Karen; Battaglia, David; Lee, David; Wu, Diana; Jensen, Jeffrey; Patton, Phillip; Gokhale, Sumita; Stouffer, Richard; Wolf, Don; Mitalipov, Shoukhrat.

In: Cell, Vol. 153, No. 6, 06.06.2013, p. 1228-1238.

Research output: Contribution to journalArticle

Tachibana, M, Amato, P, Sparman, M, Gutierrez, NM, Tippner-Hedges, R, Ma, H, Kang, E, Fulati, A, Lee, HS, Sritanaudomchai, H, Masterson, K, Larson, J, Eaton, D, Sadler-Fredd, K, Battaglia, D, Lee, D, Wu, D, Jensen, J, Patton, P, Gokhale, S, Stouffer, R, Wolf, D & Mitalipov, S 2013, 'Human embryonic stem cells derived by somatic cell nuclear transfer', Cell, vol. 153, no. 6, pp. 1228-1238. https://doi.org/10.1016/j.cell.2013.05.006
Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-Hedges R, Ma H et al. Human embryonic stem cells derived by somatic cell nuclear transfer. Cell. 2013 Jun 6;153(6):1228-1238. https://doi.org/10.1016/j.cell.2013.05.006
Tachibana, Masahito ; Amato, Paula ; Sparman, Michelle ; Gutierrez, Nuria Marti ; Tippner-Hedges, Rebecca ; Ma, Hong ; Kang, Eunju ; Fulati, Alimujiang ; Lee, Hyo Sang ; Sritanaudomchai, Hathaitip ; Masterson, Keith ; Larson, Janine ; Eaton, Deborah ; Sadler-Fredd, Karen ; Battaglia, David ; Lee, David ; Wu, Diana ; Jensen, Jeffrey ; Patton, Phillip ; Gokhale, Sumita ; Stouffer, Richard ; Wolf, Don ; Mitalipov, Shoukhrat. / Human embryonic stem cells derived by somatic cell nuclear transfer. In: Cell. 2013 ; Vol. 153, No. 6. pp. 1228-1238.
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