TY - JOUR
T1 - Human embryonic stem cells derived by somatic cell nuclear transfer
AU - Tachibana, Masahito
AU - Amato, Paula
AU - Sparman, Michelle
AU - Gutierrez, Nuria Marti
AU - Tippner-Hedges, Rebecca
AU - Ma, Hong
AU - Kang, Eunju
AU - Fulati, Alimujiang
AU - Lee, Hyo Sang
AU - Sritanaudomchai, Hathaitip
AU - Masterson, Keith
AU - Larson, Janine
AU - Eaton, Deborah
AU - Sadler-Fredd, Karen
AU - Battaglia, David
AU - Lee, David
AU - Wu, Diana
AU - Jensen, Jeffrey
AU - Patton, Phillip
AU - Gokhale, Sumita
AU - Stouffer, Richard L.
AU - Wolf, Don
AU - Mitalipov, Shoukhrat
N1 - Funding Information:
The authors would like to acknowledge the OHSU Embryonic Stem Cell Research Oversight Committee and the Institutional Review Board for providing oversight and guidance with human embryo and ESC studies. We thank egg donor volunteers and the staff at the Women’s Health Research Unit at the Center for Women’s Health, the Division of Reproductive Endocrinology & Infertility of the Department of Obstetrics & Gynecology of Oregon Health & Science University for their support and procurement of human gametes. The Division of Animal Resources, Surgery Team, Assisted Reproductive Technology & Embryonic Stem Cell Core, Endocrine Technology Core, and Imaging & Morphology Core at the Oregon National Primate Research Center provided expertise and services for the nonhuman primate studies. Hamilton Thorne donated the XYClone laser system for this study. We are grateful to Dr. Warren Sanger and Dianna Zaleski for karyotyping services, Dr. Cecilia Penedo for microsatellite analysis, and Dr. Cary Harding for assistance with nuclear donor cells. We are also indebted to Vanessa Domush, Elizabeth Smolens, Cathy Ramsey, Ying Li, Riffat Ahmed, Brittany Daughtry, and Erin Wolff for their technical support. The human oocyte/embryo research was supported by OHSU institutional funds and by the grant from Leducq Foundation. The nonhuman primate studies were supported by grants from the National Institutes of Health HD063276, HD057121, HD059946, EY021214, and 8P51OD011092.
PY - 2013/6/6
Y1 - 2013/6/6
N2 - Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. PaperClip
AB - Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. PaperClip
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U2 - 10.1016/j.cell.2013.05.006
DO - 10.1016/j.cell.2013.05.006
M3 - Article
C2 - 23683578
AN - SCOPUS:84878838747
SN - 0092-8674
VL - 153
SP - 1228
EP - 1238
JO - Cell
JF - Cell
IS - 6
ER -