Human cytomegalovirus US2 causes similar effects on both major histocompatibility complex class I and II proteins in epithelial and glial cells

Nagendra R. Hegde, David Johnson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The human cytomegalovirus (HCMV) glycoprotein US2 specifically binds to major histocompatibility complex (MHC) class I heavy chain (HC) and class II proteins DRα and DMα, triggering their degradation by proteasomes. Effects of US2 on class II proteins were originally characterized in HCMV- or adenovirus vector-infected U373 astroglioma cells. Here, we have extended characterization of US2-mediated degradation of class II DRα to two other cell lines, including biologically relevant epithelial cells. Comparison of the effects of US2 in cells expressing both class I and II proteins demonstrated only a slight preference for class I HC. Moreover, US2 caused degradation of DRα and DMα when these proteins were expressed by transfection without DRβ, invariant chain (Ii), or DMβ. Therefore, US2 binds to α chains of DR and DM and triggers endoplasmic reticulum degradation without formation of class II DR αβ/Ii or DM αβ complexes. Similar levels of degradation of class II α were observed in cells expressing vastly different amounts of class II, suggesting that cellular factors, other than class II, were limiting. We concluded that US2 has broad effects in a variety of cells that express both class I and II proteins and is relevant to HCMV infection in vivo.

Original languageEnglish (US)
Pages (from-to)9287-9294
Number of pages8
JournalJournal of Virology
Volume77
Issue number17
DOIs
StatePublished - Sep 1 2003

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Human herpesvirus 5
neuroglia
major histocompatibility complex
Major Histocompatibility Complex
Cytomegalovirus
Neuroglia
epithelial cells
Epithelial Cells
degradation
Proteins
proteins
cells
Astrocytoma
Cytomegalovirus Infections
proteasome endopeptidase complex
Adenoviridae
Proteasome Endopeptidase Complex
transfection
Endoplasmic Reticulum
endoplasmic reticulum

ASJC Scopus subject areas

  • Immunology

Cite this

Human cytomegalovirus US2 causes similar effects on both major histocompatibility complex class I and II proteins in epithelial and glial cells. / Hegde, Nagendra R.; Johnson, David.

In: Journal of Virology, Vol. 77, No. 17, 01.09.2003, p. 9287-9294.

Research output: Contribution to journalArticle

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