Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes

Mark N. Prichard, Elizabeth Sztul, Shannon L. Daily, Amie L. Perry, Samuel L. Frederick, Rachel B. Gill, Caroll B. Hartline, Daniel Streblow, Susan M. Varnum, Richard D. Smith, Earl R. Kern

Research output: Contribution to journalArticle

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Abstract

Cells infected with human cytomegalovirus in the absence of UL97 kinase activity produce large nuclear aggregates that sequester considerable quantities of viral proteins. A transient expression assay suggested that pp71 and IE1 were also involved in this process, and this suggestion was significant, since both proteins have been reported to interact with components of promyelocytic leukemia (PML) bodies (ND10) and also interact functionally with retinoblastoma pocket proteins (RB). PML bodies have been linked to the formation of nuclear aggresomes, and colocalization studies suggested that viral proteins were recruited to these structures and that UL97 kinase activity inhibited their formation. Proteins associated with PML bodies were examined by Western blot analysis, and pUL97 appeared to specifically affect the phosphorylation of RB in a kinase-dependent manner. Three consensus RB binding motifs were identified in the UL97 kinase, and recombinant viruses were constructed in which each was mutated to assess a potential role in the phosphorylation of RB and the inhibition of nuclear aggresome formation. The mutation of either the conserved LxCxE RB binding motif or the lysine required for kinase activity impaired the ability of the virus to stabilize and phosphorylate RB. We concluded from these studies that both UL97 kinase activity and the LxCxE RB binding motif are required for the phosphorylation and stabilization of RB in infected cells and that this effect can be antagonized by the antiviral drug maribavir. These data also suggest a potential link between RB function and the formation of aggresomes.

Original languageEnglish (US)
Pages (from-to)5054-5067
Number of pages14
JournalJournal of Virology
Volume82
Issue number10
DOIs
StatePublished - May 2008

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Human herpesvirus 5
Retinoblastoma Protein
Cytomegalovirus
phosphotransferases (kinases)
Phosphotransferases
leukemia
phosphorylation
Leukemia
proteins
viral proteins
Phosphorylation
Viral Proteins
antiviral agents
Viruses
viruses
protein aggregates
Lysine
Antiviral Agents
lysine
Western blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes. / Prichard, Mark N.; Sztul, Elizabeth; Daily, Shannon L.; Perry, Amie L.; Frederick, Samuel L.; Gill, Rachel B.; Hartline, Caroll B.; Streblow, Daniel; Varnum, Susan M.; Smith, Richard D.; Kern, Earl R.

In: Journal of Virology, Vol. 82, No. 10, 05.2008, p. 5054-5067.

Research output: Contribution to journalArticle

Prichard, MN, Sztul, E, Daily, SL, Perry, AL, Frederick, SL, Gill, RB, Hartline, CB, Streblow, D, Varnum, SM, Smith, RD & Kern, ER 2008, 'Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes', Journal of Virology, vol. 82, no. 10, pp. 5054-5067. https://doi.org/10.1128/JVI.02174-07
Prichard, Mark N. ; Sztul, Elizabeth ; Daily, Shannon L. ; Perry, Amie L. ; Frederick, Samuel L. ; Gill, Rachel B. ; Hartline, Caroll B. ; Streblow, Daniel ; Varnum, Susan M. ; Smith, Richard D. ; Kern, Earl R. / Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes. In: Journal of Virology. 2008 ; Vol. 82, No. 10. pp. 5054-5067.
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