Human cytomegalovirus productively infects primary differentiated macrophages

Carlos E. Ibanez, Rachel Schrier, Peter Ghazal, Clayton Wiley, Jay Nelson

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

Monocytes are one of the predominant cell types in the peripheral blood that are infected by human cytomegalovirus (HCMV). Although virus can be detected in these cells in vivo, HCMV replication in cultured monocytes has been unsuccessful. In this study, we demonstrate efficient HCMV replication in cultured monocytes. HCMV permissiveness in these cells was dependent on nonadherent cell-induced stimulation of the monocyte, with subsequent morphological differentiation into macrophages. Approximately 40% of the cells infected by virus were detected by immunofluorescent staining with both immediate-early and late antibodies. In addition, viral plaque assays demonstrated significant productive infection of macrophages. These observations are consistent with the suggestion that the monocyte/macrophage serves as a source of viral amplification and dissemination.

Original languageEnglish (US)
Pages (from-to)6581-6588
Number of pages8
JournalJournal of Virology
Volume65
Issue number12
StatePublished - Dec 1991
Externally publishedYes

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Human herpesvirus 5
Cytomegalovirus
monocytes
Monocytes
macrophages
Macrophages
Viral Plaque Assay
cells
Permissiveness
Viruses
viruses
Staining and Labeling
antibodies
Antibodies
blood
assays
Infection
infection

ASJC Scopus subject areas

  • Immunology

Cite this

Ibanez, C. E., Schrier, R., Ghazal, P., Wiley, C., & Nelson, J. (1991). Human cytomegalovirus productively infects primary differentiated macrophages. Journal of Virology, 65(12), 6581-6588.

Human cytomegalovirus productively infects primary differentiated macrophages. / Ibanez, Carlos E.; Schrier, Rachel; Ghazal, Peter; Wiley, Clayton; Nelson, Jay.

In: Journal of Virology, Vol. 65, No. 12, 12.1991, p. 6581-6588.

Research output: Contribution to journalArticle

Ibanez, CE, Schrier, R, Ghazal, P, Wiley, C & Nelson, J 1991, 'Human cytomegalovirus productively infects primary differentiated macrophages', Journal of Virology, vol. 65, no. 12, pp. 6581-6588.
Ibanez CE, Schrier R, Ghazal P, Wiley C, Nelson J. Human cytomegalovirus productively infects primary differentiated macrophages. Journal of Virology. 1991 Dec;65(12):6581-6588.
Ibanez, Carlos E. ; Schrier, Rachel ; Ghazal, Peter ; Wiley, Clayton ; Nelson, Jay. / Human cytomegalovirus productively infects primary differentiated macrophages. In: Journal of Virology. 1991 ; Vol. 65, No. 12. pp. 6581-6588.
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