Human cytomegalovirus persistently infects aortic endothelial cells

K. N. Fish, C. Soderberg-Naucler, L. K. Mills, S. Stenglein, Jay Nelson

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Endothelial cells (EC) have been implicated as constituting an important cell type in the pathogenesis of human cytomegalovirus (HCMV). Microvascular and macrovascular EC exhibit different biochemical and functional properties depending on the organ of origin. Phenotypic differences between microvascular and macrovascular EC may alter the ability of these cells to support HCMV replication. In this study, we compared the replication of HCMV in primary macrovascular aortic EC (AEC) with that in brain microvascular EC (BMVEC). An examination of IE72, pp65, and gB viral antigen expression in BMVEC and AEC by immunoflourescence revealed similar frequencies of infected cells. Intracellular production of virus was 3 log units greater in BMVEC than in AEC, while equal quantities of extracellular virus were produced in both cell types. HCMV infection of BMVEC resulted in rapid cellular lysis, while the virus was nonlytic and continuously released from HCMV-infected AEC for the life span of the culture. An examination of infected cells by electron microscopy revealed the formation of abundant nucleocapsids in both AEC and BMVEC. However, significant amounts of mature viral particles were only detected in the cytoplasm of BMVEC. These observations indicate that levels of HCMV replication in EC obtained from different organs are distinct and suggest that persistently infected AEC may serve as a reservoir of virus.

Original languageEnglish (US)
Pages (from-to)5661-5668
Number of pages8
JournalJournal of Virology
Volume72
Issue number7
StatePublished - 1998

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Human herpesvirus 5
Cytomegalovirus
endothelial cells
Endothelial Cells
brain
Brain
Viruses
viruses
cells
Nucleocapsid
nucleocapsid
Viral Antigens
viral antigens
Cytomegalovirus Infections
virion
Virion
functional properties
electron microscopy
Electron Microscopy
Cytoplasm

ASJC Scopus subject areas

  • Immunology

Cite this

Fish, K. N., Soderberg-Naucler, C., Mills, L. K., Stenglein, S., & Nelson, J. (1998). Human cytomegalovirus persistently infects aortic endothelial cells. Journal of Virology, 72(7), 5661-5668.

Human cytomegalovirus persistently infects aortic endothelial cells. / Fish, K. N.; Soderberg-Naucler, C.; Mills, L. K.; Stenglein, S.; Nelson, Jay.

In: Journal of Virology, Vol. 72, No. 7, 1998, p. 5661-5668.

Research output: Contribution to journalArticle

Fish, KN, Soderberg-Naucler, C, Mills, LK, Stenglein, S & Nelson, J 1998, 'Human cytomegalovirus persistently infects aortic endothelial cells', Journal of Virology, vol. 72, no. 7, pp. 5661-5668.
Fish KN, Soderberg-Naucler C, Mills LK, Stenglein S, Nelson J. Human cytomegalovirus persistently infects aortic endothelial cells. Journal of Virology. 1998;72(7):5661-5668.
Fish, K. N. ; Soderberg-Naucler, C. ; Mills, L. K. ; Stenglein, S. ; Nelson, Jay. / Human cytomegalovirus persistently infects aortic endothelial cells. In: Journal of Virology. 1998 ; Vol. 72, No. 7. pp. 5661-5668.
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