Human Cytomegalovirus Inhibits Tapasin-Dependent Peptide Loading and Optimization of the MHC Class I Peptide Cargo for Immune Evasion

Boyoun Park, Youngkyun Kim, Jinwook Shin, Sunray Lee, Kwangmin Cho, Klaus Frueh, Sungwook Lee, Kwangseog Ahn

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The immune evasion protein US3 of human cytomegalovirus binds to and arrests MHC class I molecules in the endoplasmic reticulum (ER). However, substantial amounts of class I molecules still escape US3-mediated ER retention, suggesting that not all class I alleles are affected equally by US3. Here, we identify tapasin inhibition as the mechanism of MHC retention by US3. US3 directly binds tapasin and inhibits tapasin-dependent peptide loading, thereby preventing the optimization of the peptide repertoire presented by class I molecules. Due to the allelic specificity of tapasin toward class I molecules, US3 affects only class I alleles that are dependent on tapasin for peptide loading and surface expression. Accordingly, tapasin-independent class I alleles selectively escape to the cell surface.

Original languageEnglish (US)
Pages (from-to)71-85
Number of pages15
JournalImmunity
Volume20
Issue number1
DOIs
StatePublished - Jan 2004

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Immune Evasion
Cytomegalovirus
Peptides
Alleles
Endoplasmic Reticulum
tapasin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Human Cytomegalovirus Inhibits Tapasin-Dependent Peptide Loading and Optimization of the MHC Class I Peptide Cargo for Immune Evasion. / Park, Boyoun; Kim, Youngkyun; Shin, Jinwook; Lee, Sunray; Cho, Kwangmin; Frueh, Klaus; Lee, Sungwook; Ahn, Kwangseog.

In: Immunity, Vol. 20, No. 1, 01.2004, p. 71-85.

Research output: Contribution to journalArticle

Park, Boyoun ; Kim, Youngkyun ; Shin, Jinwook ; Lee, Sunray ; Cho, Kwangmin ; Frueh, Klaus ; Lee, Sungwook ; Ahn, Kwangseog. / Human Cytomegalovirus Inhibits Tapasin-Dependent Peptide Loading and Optimization of the MHC Class I Peptide Cargo for Immune Evasion. In: Immunity. 2004 ; Vol. 20, No. 1. pp. 71-85.
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