Human cytomegalovirus induces cellular and humoral virus-specific immune responses in humanized BLT mice

Lindsey B. Crawford, Rebecca Tempel, Daniel Streblow, Craig Kreklywich, Patricia Smith, Louis Picker, Jay Nelson, Patrizia Caposio

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mice (huNSG) results in viral latency that can be reactivated following G-CSF treatment. In this study, we characterized the functional human adaptive immune responses in HCMV latently-infected huBLT (humanized Bone marrow-Liver-Thymus) mice. Following infection, huBLT mice generate human effector and central memory CD4+ and CD8+ T-cell responses reactive to peptides corresponding to both IE and pp65 proteins. Additionally, both HCMV specific IgM and IgG B-cell responses with the ability to neutralize virus were detected. These results indicate that the HCMV huBLT mouse model may provide a valuable tool to study viral latency and reactivation as well as evaluate HCMV vaccines and immune responses in the context of a functional human immune system.

Original languageEnglish (US)
Article number937
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Human cytomegalovirus induces cellular and humoral virus-specific immune responses in humanized BLT mice'. Together they form a unique fingerprint.

  • Cite this