Human cytomegalovirus induces cellular and humoral virus-specific immune responses in humanized BLT mice

Lindsey B. Crawford, Rebecca Tempel, Daniel Streblow, Craig Kreklywich, Patricia Smith, Louis Picker, Jay Nelson, Patrizia Caposio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mice (huNSG) results in viral latency that can be reactivated following G-CSF treatment. In this study, we characterized the functional human adaptive immune responses in HCMV latently-infected huBLT (humanized Bone marrow-Liver-Thymus) mice. Following infection, huBLT mice generate human effector and central memory CD4+ and CD8+ T-cell responses reactive to peptides corresponding to both IE and pp65 proteins. Additionally, both HCMV specific IgM and IgG B-cell responses with the ability to neutralize virus were detected. These results indicate that the HCMV huBLT mouse model may provide a valuable tool to study viral latency and reactivation as well as evaluate HCMV vaccines and immune responses in the context of a functional human immune system.

Original languageEnglish (US)
Article number937
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Cytomegalovirus
Viruses
Virus Latency
Thymus Gland
Bone Marrow
Adaptive Immunity
Liver
Immune System
Cytomegalovirus Vaccines
Species Specificity
Cytomegalovirus Infections
Granulocyte Colony-Stimulating Factor
Hematopoietic Stem Cells
Antiviral Agents
Immunoglobulin M
B-Lymphocytes
Immunoglobulin G
T-Lymphocytes
Peptides

ASJC Scopus subject areas

  • General

Cite this

Human cytomegalovirus induces cellular and humoral virus-specific immune responses in humanized BLT mice. / Crawford, Lindsey B.; Tempel, Rebecca; Streblow, Daniel; Kreklywich, Craig; Smith, Patricia; Picker, Louis; Nelson, Jay; Caposio, Patrizia.

In: Scientific Reports, Vol. 7, No. 1, 937, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Picker, Louis

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