Human cytomegalovirus glycoprotein N (gpUL73-gN) genomic variants: Identification of a novel subgroup, geographical distribution and evidence of positive selective pressure

S. Pignatelli, Paola Dal Monte, G. Rossini, Sunwen Chou, T. Gojobori, K. Hanada, J. J. Guo, W. Rawlinson, W. Britt, M. Mach, M. P. Landini

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) ORF UL73 is a polymorphic locus, encoding the viral glycoprotein gpUL73-gN, a component of the gC-II envelope complex. The previously identified gN genomic variants, denoted gN-1, gN-2, gN-3 and gN-4, were further investigated in this work by analysing a large panel of HCMV clinical isolates collected from all over the world (223 samples). Sequencing and phylogenetic analysis confirmed the existence of the four gN genotypes, but also allowed the identification of a novel subgroup belonging to the gN-3 genotype, which was designated gN-3b. The number of non-synonymous (dN) and synonymous (dS) nucleotide substitutions and their ratio (dN/dS) were estimated among the gN genotypes to evaluate the possibility of positive selection. Results showed that the four variants evolved by neutral (random) selection, but that the gN-3 and gN-4 genotypes are maintained by positive selective pressure. The 223 HCMV clinical isolates were subdivided according to their geographical origin, and four main regions of gN prevalence were identified: Europe, China, Australia and Northern America. The gN variants were found to be widespread and represented within the regions analysed without any significant difference, and no new genotype was detected. Finally, for clinical and epidemiological purposes, a rapid and low-cost method for genetic grouping of the HCMV clinical isolates was developed based on the RFLP revealed by Sacl, Scal and Sa/l digestion of the PCR-amplified UL73 sequence. This technique enabled us to distinguish all four gN genomic variants and also their subtypes.

Original languageEnglish (US)
Pages (from-to)647-655
Number of pages9
JournalJournal of General Virology
Volume84
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

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Cytomegalovirus
Genotype
Medical Genetics
North America
Restriction Fragment Length Polymorphisms
Open Reading Frames
Digestion
China
Glycoproteins
Nucleotides
Human cytomegalovirus glycoprotein N
Costs and Cost Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Human cytomegalovirus glycoprotein N (gpUL73-gN) genomic variants : Identification of a novel subgroup, geographical distribution and evidence of positive selective pressure. / Pignatelli, S.; Dal Monte, Paola; Rossini, G.; Chou, Sunwen; Gojobori, T.; Hanada, K.; Guo, J. J.; Rawlinson, W.; Britt, W.; Mach, M.; Landini, M. P.

In: Journal of General Virology, Vol. 84, No. 3, 01.03.2003, p. 647-655.

Research output: Contribution to journalArticle

Pignatelli, S. ; Dal Monte, Paola ; Rossini, G. ; Chou, Sunwen ; Gojobori, T. ; Hanada, K. ; Guo, J. J. ; Rawlinson, W. ; Britt, W. ; Mach, M. ; Landini, M. P. / Human cytomegalovirus glycoprotein N (gpUL73-gN) genomic variants : Identification of a novel subgroup, geographical distribution and evidence of positive selective pressure. In: Journal of General Virology. 2003 ; Vol. 84, No. 3. pp. 647-655.
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