Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family

Roque D. Carrero-Valenzuela; Franklin Quan; Robert Lightowlers; Nancy G. Kennaway; Michael Litt; Michael Forte

doi:10.1016/0378-1119(91)90082-M

Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family

Roque D. Carrero-Valenzuela, Franklin Quan, Robert Lightowlers, Nancy G. Kennaway, Michael Litt, Michael Forte

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit Vlb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.

Original language	English (US)
Pages (from-to)	229-236
Number of pages	8
Journal	Gene
Volume	102
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(91)90082-M
State	Published - Jun 30 1991
Externally published	Yes

Keywords

A phage vector
Recombinant DNA
cDNA cloning
multiple loci
pseudogenes

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90082-M

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@article{09b8d2a219bb4c6ba8d74196a78e4935,

title = "Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family",

abstract = "We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit Vlb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.",

keywords = "A phage vector, Recombinant DNA, cDNA cloning, multiple loci, pseudogenes",

author = "Carrero-Valenzuela, {Roque D.} and Franklin Quan and Robert Lightowlers and Kennaway, {Nancy G.} and Michael Litt and Michael Forte",

note = "Funding Information: We are grateful to Dr. T. Hefty, Director, Pacific North-west Transplant Bank, for his help in acquiring human tissues and to Ms. Linda Nay for preparing the manuscript. We thank T.K. Mohandas for providing DNAs from a somatic cell hybrid mapping panel. This work was supported by grants from the Muscular Dystrophy Association and the Medical Research Foundation of Oregon; R.D.C.-V. was supported in part by a Tartar Research Fellowship and a Post-Doctoral Fellowship from the Oregon Health Sciences University. F.Q. is the recipient of a Medical Research Council (Canada) Postdoctoral Fellowship.",

year = "1991",

month = jun,

day = "30",

doi = "10.1016/0378-1119(91)90082-M",

language = "English (US)",

volume = "102",

pages = "229--236",

journal = "Gene",

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T1 - Human cytochrome c oxidase subunit VIb

T2 - characterization and mapping of a multigene family

AU - Carrero-Valenzuela, Roque D.

AU - Quan, Franklin

AU - Lightowlers, Robert

AU - Kennaway, Nancy G.

AU - Litt, Michael

AU - Forte, Michael

N1 - Funding Information: We are grateful to Dr. T. Hefty, Director, Pacific North-west Transplant Bank, for his help in acquiring human tissues and to Ms. Linda Nay for preparing the manuscript. We thank T.K. Mohandas for providing DNAs from a somatic cell hybrid mapping panel. This work was supported by grants from the Muscular Dystrophy Association and the Medical Research Foundation of Oregon; R.D.C.-V. was supported in part by a Tartar Research Fellowship and a Post-Doctoral Fellowship from the Oregon Health Sciences University. F.Q. is the recipient of a Medical Research Council (Canada) Postdoctoral Fellowship.

PY - 1991/6/30

Y1 - 1991/6/30

N2 - We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit Vlb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.

AB - We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit Vlb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.

KW - A phage vector

KW - Recombinant DNA

KW - cDNA cloning

KW - multiple loci

KW - pseudogenes

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JO - Gene

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Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family

Abstract

Keywords

ASJC Scopus subject areas

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