Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family

Roque D. Carrero-Valenzuela, Franklin Quan, Robert Lightowlers, Nancy G. Kennaway, Michael Litt, Michael Forte

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit Vlb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalGene
Volume102
Issue number2
DOIs
StatePublished - Jun 30 1991

Keywords

  • A phage vector
  • Recombinant DNA
  • cDNA cloning
  • multiple loci
  • pseudogenes

ASJC Scopus subject areas

  • Genetics

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