Human CLP1 mutations alter tRNA biogenesis, Affecting both peripheral and central nervous system function

Ender Karaca, Stefan Weitzer, Davut Pehlivan, Hiroshi Shiraishi, Tasos Gogakos, Toshikatsu Hanada, Shalini N. Jhangiani, Wojciech Wiszniewski, Marjorie Withers, Ian M. Campbell, Serkan Erdin, Sedat Isikay, Luis M. Franco, Claudia Gonzaga-Jauregui, Tomasz Gambin, Violet Gelowani, Jill V. Hunter, Gozde Yesil, Erkan Koparir, Sarenur YilmazMiguel Brown, Daniel Briskin, Markus Hafner, Pavel Morozov, Thalia A. Farazi, Christian Bernreuther, Markus Glatzel, Siegfried Trattnig, Joachim Friske, Claudia Kronnerwetter, Matthew N. Bainbridge, Alper Gezdirici, Mehmet Seven, Donna M. Muzny, Eric Boerwinkle, Mustafa Ozen, Tim Clausen, Thomas Tuschl, Adnan Yuksel, Andreas Hess, Richard A. Gibbs, Javier Martinez, Josef M. Penninger, James R. Lupski

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

CLP1 is a RNA kinase involved in tRNA splicing. Recently, CLP1 kinase-dead mice were shown to display a neuromuscular disorder with loss of motor neurons and muscle paralysis. Human genome analyses now identified a CLP1 homozygous missense mutation (p.R140H) in five unrelated families, leading to a loss of CLP1 interaction with the tRNA splicing endonuclease (TSEN) complex, largely reduced pre-tRNA cleavage activity, and accumulation of linear tRNA introns. The affected individuals develop severe motor-sensory defects, cortical dysgenesis, and microcephaly. Mice carrying kinase-dead CLP1 also displayed microcephaly and reduced cortical brain volume due to the enhanced cell death of neuronal progenitors that is associated with reduced numbers of cortical neurons. Our data elucidate a neurological syndrome defined by CLP1 mutations that impair tRNA splicing. Reduction of a founder mutation to homozygosity illustrates the importance of rare variations in disease and supports the clan genomics hypothesis.

Original languageEnglish (US)
Pages (from-to)636-650
Number of pages15
JournalCell
Volume157
Issue number3
DOIs
StatePublished - Apr 24 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Karaca, E., Weitzer, S., Pehlivan, D., Shiraishi, H., Gogakos, T., Hanada, T., Jhangiani, S. N., Wiszniewski, W., Withers, M., Campbell, I. M., Erdin, S., Isikay, S., Franco, L. M., Gonzaga-Jauregui, C., Gambin, T., Gelowani, V., Hunter, J. V., Yesil, G., Koparir, E., ... Lupski, J. R. (2014). Human CLP1 mutations alter tRNA biogenesis, Affecting both peripheral and central nervous system function. Cell, 157(3), 636-650. https://doi.org/10.1016/j.cell.2014.02.058