Human B cell lines secreting IgM antibody specific for myelin basic protein

Zhang Jingwu, Jos Lambrechts, Harry Heyligen, Arthur A. Vandenbark, Jef C.M. Raus

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5-12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalJournal of Neuroimmunology
Volume24
Issue number1-2
DOIs
StatePublished - Sep 1989

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Keywords

  • Epstein-Barr virus transformation
  • Immunoglubin M-secreting B lymphoblastoid cell line
  • Myelin basic protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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