Horseradish peroxidase acute ototoxicity and the uptake and movement of the peroxidase in the auditory system of the Guinea pig

Muriel D. Ross, Alfred Nuttall, Ch G. Wright

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

When guinea pig cochlea was perfused in vivo with a solution of 1 % horseradish peroxidase (HRP) in artificial perilymph, the enzyme was found in the basilar membrane, spiral limbus, some outer and inner hair cells and some supporting cells and it was gradually cleared away with time. Acute signs of cell damage included swelling, vacuolization and diffuse labeling of some hair cells, but stereocilia remained normal in configuration. Albino melanocytes of the spiral ligament were also damaged, and vacuolization of Reissner's membrane occurred after 10% HRP. Both concentrations caused a gradual decline in CM, showing that HRP is acutely oto-toxic but its mode of action is unknown. No retrograde transport of HRP to spiral ganglion cells or to brain stem neurons occurred, but some brain stem neurons took up HRP from the neuropil following diffusion from the cochlea.

Original languageEnglish (US)
Pages (from-to)187-201
Number of pages15
JournalActa Oto-Laryngologica
Volume84
Issue number1-6
DOIs
StatePublished - 1977
Externally publishedYes

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Horseradish Peroxidase
Peroxidase
Guinea Pigs
Cochlea
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Brain Stem
Spiral Ligament of Cochlea
Basilar Membrane
Stereocilia
Perilymph
Spiral Ganglion
Neurons
Neuropil
Poisons
Melanocytes
Membranes
Enzymes

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Horseradish peroxidase acute ototoxicity and the uptake and movement of the peroxidase in the auditory system of the Guinea pig. / Ross, Muriel D.; Nuttall, Alfred; Wright, Ch G.

In: Acta Oto-Laryngologica, Vol. 84, No. 1-6, 1977, p. 187-201.

Research output: Contribution to journalArticle

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