Hormonal regulation of prostaglandin E2 receptors: Localization and expression in rat cervical tissue

Andrea C. Hinton; Peta L. Grigsby; Bradley A. Pitzer; Diane E. Brockman; Richard F. Ittenbach; Robert B. Hinton; Leslie Myatt

doi:10.1177/1933719109348068

Hormonal regulation of prostaglandin E2 receptors: Localization and expression in rat cervical tissue

Andrea C. Hinton, Peta L. Grigsby, Bradley A. Pitzer, Diane E. Brockman, Richard F. Ittenbach, Robert B. Hinton, Leslie Myatt

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Prostaglandin E2 (PGE2) may regulate uterine activation and cervical ripening for labor through specific contractile and relaxatory receptors (EP1-4). The aim of this study was to determine the expression of PGE2 receptor isoforms in pregnant rat cervix during RU486-induced labor and progesterone supplementation to delay labor. Localization and expression of cervical PGE2 receptors were evaluated, and quantitative real-time polymerase chain reaction (PCR) for EP1-4 was performed. EP1-4 were found in both cervical epithelium and smooth muscle. RU486 treatment increased EP2 and EP4 messenger RNA (mRNA) and protein expression. Progesterone treatment had no effect on EP2 and EP4 mRNA expression but decreased EP4 protein. Hormonal manipulation resulted in differences in cellular localization of EP1 and EP3 in cervical epithelial cells, suggesting a specific role in that cell. Progesterone differentially regulates the expression of PGE2 receptor isoforms in the cervix. Elucidating the regulation of PGE2 receptors may facilitate improved approaches to the prevention and treatment of preterm labor.

Original language	English (US)
Pages (from-to)	136-146
Number of pages	11
Journal	Reproductive Sciences
Volume	17
Issue number	2
DOIs	https://doi.org/10.1177/1933719109348068
State	Published - Feb 2010
Externally published	Yes

Keywords

Cervix
Mifepristone
Progesterone
Prostaglandin receptors

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1177/1933719109348068

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title = "Hormonal regulation of prostaglandin E2 receptors: Localization and expression in rat cervical tissue",

abstract = "Prostaglandin E2 (PGE2) may regulate uterine activation and cervical ripening for labor through specific contractile and relaxatory receptors (EP1-4). The aim of this study was to determine the expression of PGE2 receptor isoforms in pregnant rat cervix during RU486-induced labor and progesterone supplementation to delay labor. Localization and expression of cervical PGE2 receptors were evaluated, and quantitative real-time polymerase chain reaction (PCR) for EP1-4 was performed. EP1-4 were found in both cervical epithelium and smooth muscle. RU486 treatment increased EP2 and EP4 messenger RNA (mRNA) and protein expression. Progesterone treatment had no effect on EP2 and EP4 mRNA expression but decreased EP4 protein. Hormonal manipulation resulted in differences in cellular localization of EP1 and EP3 in cervical epithelial cells, suggesting a specific role in that cell. Progesterone differentially regulates the expression of PGE2 receptor isoforms in the cervix. Elucidating the regulation of PGE2 receptors may facilitate improved approaches to the prevention and treatment of preterm labor.",

keywords = "Cervix, Mifepristone, Progesterone, Prostaglandin receptors",

author = "Hinton, {Andrea C.} and Grigsby, {Peta L.} and Pitzer, {Bradley A.} and Brockman, {Diane E.} and Ittenbach, {Richard F.} and Hinton, {Robert B.} and Leslie Myatt",

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AU - Grigsby, Peta L.

AU - Pitzer, Bradley A.

AU - Brockman, Diane E.

AU - Ittenbach, Richard F.

AU - Hinton, Robert B.

AU - Myatt, Leslie

PY - 2010/2

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N2 - Prostaglandin E2 (PGE2) may regulate uterine activation and cervical ripening for labor through specific contractile and relaxatory receptors (EP1-4). The aim of this study was to determine the expression of PGE2 receptor isoforms in pregnant rat cervix during RU486-induced labor and progesterone supplementation to delay labor. Localization and expression of cervical PGE2 receptors were evaluated, and quantitative real-time polymerase chain reaction (PCR) for EP1-4 was performed. EP1-4 were found in both cervical epithelium and smooth muscle. RU486 treatment increased EP2 and EP4 messenger RNA (mRNA) and protein expression. Progesterone treatment had no effect on EP2 and EP4 mRNA expression but decreased EP4 protein. Hormonal manipulation resulted in differences in cellular localization of EP1 and EP3 in cervical epithelial cells, suggesting a specific role in that cell. Progesterone differentially regulates the expression of PGE2 receptor isoforms in the cervix. Elucidating the regulation of PGE2 receptors may facilitate improved approaches to the prevention and treatment of preterm labor.

AB - Prostaglandin E2 (PGE2) may regulate uterine activation and cervical ripening for labor through specific contractile and relaxatory receptors (EP1-4). The aim of this study was to determine the expression of PGE2 receptor isoforms in pregnant rat cervix during RU486-induced labor and progesterone supplementation to delay labor. Localization and expression of cervical PGE2 receptors were evaluated, and quantitative real-time polymerase chain reaction (PCR) for EP1-4 was performed. EP1-4 were found in both cervical epithelium and smooth muscle. RU486 treatment increased EP2 and EP4 messenger RNA (mRNA) and protein expression. Progesterone treatment had no effect on EP2 and EP4 mRNA expression but decreased EP4 protein. Hormonal manipulation resulted in differences in cellular localization of EP1 and EP3 in cervical epithelial cells, suggesting a specific role in that cell. Progesterone differentially regulates the expression of PGE2 receptor isoforms in the cervix. Elucidating the regulation of PGE2 receptors may facilitate improved approaches to the prevention and treatment of preterm labor.

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KW - Progesterone

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Hormonal regulation of prostaglandin E2 receptors: Localization and expression in rat cervical tissue

Abstract

Keywords

ASJC Scopus subject areas

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