Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Ov Slayden, Francis Kathryn E. Friason, Kise Rosen Bond, Emily Cathleen Mishler

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    Abstract

    Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P < .05). LNG also reduced OVGP1 expression (P < .05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

    Original languageEnglish (US)
    JournalJournal of Medical Primatology
    DOIs
    Publication statusAccepted/In press - Jan 1 2018

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    Keywords

    • Cervix
    • Cytobrush
    • Hormonal regulation
    • Oviductal glycoprotein
    • Progestogen

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

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