Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Ov Slayden, Francis Kathryn E. Friason, Kise Rosen Bond, Emily Cathleen Mishler

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P < .05). LNG also reduced OVGP1 expression (P < .05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

    Original languageEnglish (US)
    JournalJournal of Medical Primatology
    DOIs
    StateAccepted/In press - Jan 1 2018

    Fingerprint

    cervix
    hormonal regulation
    Macaca mulatta
    Cervix Uteri
    glycoproteins
    Progestins
    Glycoproteins
    contraceptives
    Contraceptive Agents
    progestational hormones
    Macaca
    Levonorgestrel
    Mucous Membrane
    Epithelial Cells
    epithelial cells
    Ovulation
    laminae (animals)
    immunocytochemistry
    Progesterone
    Real-Time Polymerase Chain Reaction

    Keywords

    • Cervix
    • Cytobrush
    • Hormonal regulation
    • Oviductal glycoprotein
    • Progestogen

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

    Cite this

    Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix. / Slayden, Ov; Friason, Francis Kathryn E.; Bond, Kise Rosen; Mishler, Emily Cathleen.

    In: Journal of Medical Primatology, 01.01.2018.

    Research output: Contribution to journalArticle

    Slayden, Ov ; Friason, Francis Kathryn E. ; Bond, Kise Rosen ; Mishler, Emily Cathleen. / Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix. In: Journal of Medical Primatology. 2018.
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    abstract = "Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P < .05). LNG also reduced OVGP1 expression (P < .05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.",
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