Homocysteine upregulates vascular cell adhesion molecule-1 expression in cultured human aortic endothelial cells and enhances monocyte adhesion

Matthew D. Silverman, Ramagopal J. Tumuluri, Mishel Davis, Gladys Lopez, James (Jim) Rosenbaum, Peter I. Lelkes

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Elevated plasma homocysteine is an independent risk factor for atherosclerosis. We hypothesized that homocysteine enhances monocyte/human aortic endothelial cell (HAEC) interactions, a pivotal early event in atherogenesis, by upregulating endothelial adhesion molecules. After incubation of cultured HAECs with reduced DL-homocysteine for up to 24 hours, adhesion of human monocytes to homocysteine-stimulated HAECs was significantly upregulated in a time- and dose-dependent fashion. Pretreatment of HAECs with 100 μmol/L homocysteine caused a 4.5-fold increase in the adhesion of normal human monocytes (P

Original languageEnglish (US)
Pages (from-to)587-592
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

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Vascular Cell Adhesion Molecule-1
Homocysteine
Monocytes
Up-Regulation
Endothelial Cells
Atherosclerosis
Cell Communication

Keywords

  • Cyclooxygenase
  • Homocysteine
  • Human aortic endothelial cells
  • Monocyte adhesion
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Homocysteine upregulates vascular cell adhesion molecule-1 expression in cultured human aortic endothelial cells and enhances monocyte adhesion. / Silverman, Matthew D.; Tumuluri, Ramagopal J.; Davis, Mishel; Lopez, Gladys; Rosenbaum, James (Jim); Lelkes, Peter I.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 22, No. 4, 2002, p. 587-592.

Research output: Contribution to journalArticle

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