HLA-C downregulation by HIV-1 adapts to host HLA genotype

Nathaniel D. Bachtel, Gisele Umviligihozo, Suzanne Pickering, Talia M. Mota, Hua Liang, Gregory Q. Del Prete, Pramita Chatterjee, Guinevere Q. Lee, Rasmi Thomas, Mark A. Brockman, Stuart Neil, Mary Carrington, Bosco Bwana, David Bangsberg, Jeffrey N. Martin, Esper G. Kallas, Camila S. Donini, Natalia B. Cerqueira, Una T. O’Doherty, Beatrice H. Hahn & 4 others R. Brad Jones, Zabrina L. Brumme, Douglas F. Nixon, Richard Apps

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

HIV-1 can downregulate HLA-C on infected cells, using the viral protein Vpu, and the magnitude of this downregulation varies widely between primary HIV-1 variants. The selection pressures that result in viral downregulation of HLA-C in some individuals, but preservation of surface HLA-C in others are not clear. To better understand viral immune evasion targeting HLA-C, we have characterized HLA-C downregulation by a range of primary HIV-1 viruses. 128 replication competent viral isolates from 19 individuals with effective anti-retroviral therapy, show that a substantial minority of individuals harbor latent reservoir virus which strongly downregulates HLA-C. Untreated infections display no change in HLA-C downregulation during the first 6 months of infection, but variation between viral quasispecies can be detected in chronic infection. Vpu molecules cloned from plasma of 195 treatment naïve individuals in chronic infection demonstrate that downregulation of HLA-C adapts to host HLA genotype. HLA-C alleles differ in the pressure they exert for downregulation, and individuals with higher levels of HLA-C expression favor greater viral downregulation of HLA-C. Studies of primary and mutant molecules identify 5 residues in the transmembrane region of Vpu, and 4 residues in the transmembrane domain of HLA-C, which determine interactions between Vpu and HLA. The observed adaptation of Vpu-mediated downregulation to host genotype indicates that HLA-C alleles differ in likelihood of mediating a CTL response that is subverted by viral downregulation, and that preservation of HLA-C expression is favored in the absence of these responses. Finding that latent reservoir viruses can downregulate HLA-C could have implications for HIV-1 cure therapy approaches in some individuals.

Original languageEnglish (US)
Article numbere1007257
JournalPLoS Pathogens
Volume14
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

HLA-C Antigens
HIV-1
Down-Regulation
Genotype
Viruses
Infection
Alleles
Immune Evasion
Pressure
Viral Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Bachtel, N. D., Umviligihozo, G., Pickering, S., Mota, T. M., Liang, H., Del Prete, G. Q., ... Apps, R. (2018). HLA-C downregulation by HIV-1 adapts to host HLA genotype. PLoS Pathogens, 14(9), [e1007257]. https://doi.org/10.1371/journal.ppat.1007257

HLA-C downregulation by HIV-1 adapts to host HLA genotype. / Bachtel, Nathaniel D.; Umviligihozo, Gisele; Pickering, Suzanne; Mota, Talia M.; Liang, Hua; Del Prete, Gregory Q.; Chatterjee, Pramita; Lee, Guinevere Q.; Thomas, Rasmi; Brockman, Mark A.; Neil, Stuart; Carrington, Mary; Bwana, Bosco; Bangsberg, David; Martin, Jeffrey N.; Kallas, Esper G.; Donini, Camila S.; Cerqueira, Natalia B.; O’Doherty, Una T.; Hahn, Beatrice H.; Jones, R. Brad; Brumme, Zabrina L.; Nixon, Douglas F.; Apps, Richard.

In: PLoS Pathogens, Vol. 14, No. 9, e1007257, 01.09.2018.

Research output: Contribution to journalArticle

Bachtel, ND, Umviligihozo, G, Pickering, S, Mota, TM, Liang, H, Del Prete, GQ, Chatterjee, P, Lee, GQ, Thomas, R, Brockman, MA, Neil, S, Carrington, M, Bwana, B, Bangsberg, D, Martin, JN, Kallas, EG, Donini, CS, Cerqueira, NB, O’Doherty, UT, Hahn, BH, Jones, RB, Brumme, ZL, Nixon, DF & Apps, R 2018, 'HLA-C downregulation by HIV-1 adapts to host HLA genotype', PLoS Pathogens, vol. 14, no. 9, e1007257. https://doi.org/10.1371/journal.ppat.1007257
Bachtel ND, Umviligihozo G, Pickering S, Mota TM, Liang H, Del Prete GQ et al. HLA-C downregulation by HIV-1 adapts to host HLA genotype. PLoS Pathogens. 2018 Sep 1;14(9). e1007257. https://doi.org/10.1371/journal.ppat.1007257
Bachtel, Nathaniel D. ; Umviligihozo, Gisele ; Pickering, Suzanne ; Mota, Talia M. ; Liang, Hua ; Del Prete, Gregory Q. ; Chatterjee, Pramita ; Lee, Guinevere Q. ; Thomas, Rasmi ; Brockman, Mark A. ; Neil, Stuart ; Carrington, Mary ; Bwana, Bosco ; Bangsberg, David ; Martin, Jeffrey N. ; Kallas, Esper G. ; Donini, Camila S. ; Cerqueira, Natalia B. ; O’Doherty, Una T. ; Hahn, Beatrice H. ; Jones, R. Brad ; Brumme, Zabrina L. ; Nixon, Douglas F. ; Apps, Richard. / HLA-C downregulation by HIV-1 adapts to host HLA genotype. In: PLoS Pathogens. 2018 ; Vol. 14, No. 9.
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AU - Bachtel, Nathaniel D.

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AU - Del Prete, Gregory Q.

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AU - Lee, Guinevere Q.

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AU - Carrington, Mary

AU - Bwana, Bosco

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AU - Kallas, Esper G.

AU - Donini, Camila S.

AU - Cerqueira, Natalia B.

AU - O’Doherty, Una T.

AU - Hahn, Beatrice H.

AU - Jones, R. Brad

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AU - Nixon, Douglas F.

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