HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats

Phoebe Lin, Mary Bach, Mark Asquith, Aaron Y. Lee, Lakshmi Akileswaran, Patrick Stauffer, Sean Davin, Yuzhen Pan, Eric Cambronne, Martha Dorris, Justine W. Debelius, Christian L. Lauber, Gail Ackermann, Yoshiki V. Baeza, Tejpal Gill, Rob Knight, Robert A. Colbert, Joel D. Taurog, Russell N. Van Gelder, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The HLA-B27 gene is a major risk factor for clinical diseases including ankylosing spondylitis, acute anterior uveitis, reactive arthritis, and psoriatic arthritis, but its mechanism of risk enhancement is not completely understood. The gut microbiome has recently been shown to influence several HLA-linked diseases. However, the role of HLA-B27 in shaping the gut microbiome has not been previously investigated. In this study, we characterize the differences in the gut microbiota mediated by the presence of the HLA-B27 gene. We identified differences in the cecal microbiota of Lewis rats transgenic for HLA-B27 and human β2-microglobulin (hβ2m), compared with wild-type Lewis rats, using biome representational in situ karyotyping (BRISK) and 16S rRNA gene sequencing. 16S sequencing revealed significant differences between transgenic animals and wild type animals by principal coordinates analysis. Further analysis of the data set revealed an increase in Prevotella spp. and a decrease in Rikenellaceae relative abundance in the transgenic animals compared to the wild type animals. By BRISK analysis, species-specific differences included an increase in Bacteroides vulgatus abundance in HLA-B27/hβ2m and hβ2m compared to wild type rats. The finding that HLA-B27 is associated with altered cecal microbiota has not been shown before and can potentially provide a better understanding of the clinical diseases associated with this gene.

Original languageEnglish (US)
Article numbere105684
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 20 2014

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Transgenic Rats
HLA-B27 Antigen
intestinal microorganisms
Rats
genetically modified organisms
transgenic animals
rats
Animals
arthritis
Genes
karyotyping
Karyotyping
Bacteroides vulgatus
Genetically Modified Animals
Wild Animals
genes
digestive system
Microbiota
Prevotella
Ecosystem

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats. / Lin, Phoebe; Bach, Mary; Asquith, Mark; Lee, Aaron Y.; Akileswaran, Lakshmi; Stauffer, Patrick; Davin, Sean; Pan, Yuzhen; Cambronne, Eric; Dorris, Martha; Debelius, Justine W.; Lauber, Christian L.; Ackermann, Gail; Baeza, Yoshiki V.; Gill, Tejpal; Knight, Rob; Colbert, Robert A.; Taurog, Joel D.; Van Gelder, Russell N.; Rosenbaum, James (Jim).

In: PLoS One, Vol. 9, No. 8, e105684, 20.08.2014.

Research output: Contribution to journalArticle

Lin, P, Bach, M, Asquith, M, Lee, AY, Akileswaran, L, Stauffer, P, Davin, S, Pan, Y, Cambronne, E, Dorris, M, Debelius, JW, Lauber, CL, Ackermann, G, Baeza, YV, Gill, T, Knight, R, Colbert, RA, Taurog, JD, Van Gelder, RN & Rosenbaum, JJ 2014, 'HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats', PLoS One, vol. 9, no. 8, e105684. https://doi.org/10.1371/journal.pone.0105684
Lin, Phoebe ; Bach, Mary ; Asquith, Mark ; Lee, Aaron Y. ; Akileswaran, Lakshmi ; Stauffer, Patrick ; Davin, Sean ; Pan, Yuzhen ; Cambronne, Eric ; Dorris, Martha ; Debelius, Justine W. ; Lauber, Christian L. ; Ackermann, Gail ; Baeza, Yoshiki V. ; Gill, Tejpal ; Knight, Rob ; Colbert, Robert A. ; Taurog, Joel D. ; Van Gelder, Russell N. ; Rosenbaum, James (Jim). / HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats. In: PLoS One. 2014 ; Vol. 9, No. 8.
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