TY - JOUR
T1 - HIV-Induced T Cell Syncytia Are Self-Perpetuating and the Primary Cause of T Cell Death in Culture
AU - Sylwester, Andrew
AU - Murphy, Seamus
AU - Shutt, Damon
AU - Soll, David R.
PY - 1997/4/15
Y1 - 1997/4/15
N2 - Prior studies suggested that induced fusion in T cell cultures infected with syncytium-inducing HIV was not the primary cause of T cell death. However, these studies failed to assess the contribution of fusion in terms of syncytium volume, rather than syncytium frequency. This question has been reassessed by monitoring the frequency, volume, rate of growth, longevity, p24 production, viral budding, and self-propagating ability of syncytia in HIV-infected SUP-T1 cell cultures and individually isolated syncytia seeded in uninfected SUP-T1 cell cultures. The results demonstrate that in these cultures syncytium formation is the principal mechanism of T cell death, syncytia are the main source of virus production, and both virus production and syncytium longevity are independent of syncytium size, but dependent on syncytium age, suggesting that both are programmed events.
AB - Prior studies suggested that induced fusion in T cell cultures infected with syncytium-inducing HIV was not the primary cause of T cell death. However, these studies failed to assess the contribution of fusion in terms of syncytium volume, rather than syncytium frequency. This question has been reassessed by monitoring the frequency, volume, rate of growth, longevity, p24 production, viral budding, and self-propagating ability of syncytia in HIV-infected SUP-T1 cell cultures and individually isolated syncytia seeded in uninfected SUP-T1 cell cultures. The results demonstrate that in these cultures syncytium formation is the principal mechanism of T cell death, syncytia are the main source of virus production, and both virus production and syncytium longevity are independent of syncytium size, but dependent on syncytium age, suggesting that both are programmed events.
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M3 - Article
C2 - 9103471
AN - SCOPUS:0031569469
SN - 0022-1767
VL - 158
SP - 3996
EP - 4007
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -