HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells

Deborah Lewinsohn, Rebecca Lines, David Lewinsohn, Stanley R. Riddell, Philip D. Greenberg, Michael Emerman, Steven R. Bartz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

HIV-1 infected persons develop a robust CTL response to HIV antigens, yet HIV-1 is able to evade this host response and successfully replicate. The mechanism(s) of evasion is not completely defined but has been suggested to include resistance of infected cells to CTL-mediated apoptosis. The HIV-1 Vpr protein induces G2 arrest by indirectly inhibiting activation of cyclin B/p34cdc2 kinase. Granzyme B, the principle mediator of CTL-induced apoptosis, prematurely activates this same kinase complex. Therefore, we assessed the susceptibility of HIV-1 infected cells to CTL-mediated apoptosis to determine whether the expression of Vpr protected the infected cells from CTL-induced apoptosis. Antigen-specific CD8+ CTL were able to induce apoptosis in HIV-1 infected cells and cells labeled with peptide corresponding to the CTL epitope with equivalent efficiency. This demonstrates that neither HIV-1 Vpr nor any other HIV protein directly inhibits CTL effector functions. Furthermore, we confirm that HIV-1 Nef is able to provide partial protection from CTL recognition of infected cells. Thus, the inability of CTL to control HIV-1 infection is likely not due to direct inhibition of CTL-mediated apoptosis.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalVirology
Volume294
Issue number1
DOIs
StatePublished - 2002

Fingerprint

HIV-1
Apoptosis
Phosphotransferases
HIV Antigens
CD8 Antigens
Human Immunodeficiency Virus Proteins
Cyclin B
Granzymes
HIV Infections
Epitopes
Peptides

Keywords

  • Apoptosis
  • CD8 T-lymphocytes
  • Cytotoxic
  • Gene products
  • HIV-1
  • T-lymphocytes
  • Vpr

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Lewinsohn, D., Lines, R., Lewinsohn, D., Riddell, S. R., Greenberg, P. D., Emerman, M., & Bartz, S. R. (2002). HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells. Virology, 294(1), 13-21. https://doi.org/10.1006/viro.2001.1294

HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells. / Lewinsohn, Deborah; Lines, Rebecca; Lewinsohn, David; Riddell, Stanley R.; Greenberg, Philip D.; Emerman, Michael; Bartz, Steven R.

In: Virology, Vol. 294, No. 1, 2002, p. 13-21.

Research output: Contribution to journalArticle

Lewinsohn, D, Lines, R, Lewinsohn, D, Riddell, SR, Greenberg, PD, Emerman, M & Bartz, SR 2002, 'HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells', Virology, vol. 294, no. 1, pp. 13-21. https://doi.org/10.1006/viro.2001.1294
Lewinsohn, Deborah ; Lines, Rebecca ; Lewinsohn, David ; Riddell, Stanley R. ; Greenberg, Philip D. ; Emerman, Michael ; Bartz, Steven R. / HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells. In: Virology. 2002 ; Vol. 294, No. 1. pp. 13-21.
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