Hiv-1 envelope subregion length variation during disease progression

Marcel Curlin, Rafael Zioni, Stephen E. Hawes, Yi Liu, Wenjie Deng, Geoffrey S. Gottlieb, Tuofu Zhu, James I. Mullins

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The V3 loop of the HIV-1 Env protein is the primary determinant of viral coreceptor usage, whereas the V1V2 loop region is thought to influence coreceptor binding and participate in shielding of neutralization-sensitive regions of the Env glycoprotein gp120 from antibody responses. The functional properties and antigenicity of V1V2 are influenced by changes in amino acid sequence, sequence length and patterns of N-linked glycosylation. However, how these polymorphisms relate to HIV pathogenesis is not fully understood. We examined 5185 HIV-1 gp120 nucleotide sequence fragments and clinical data from 154 individuals (152 were infected with HIV-1 Subtype B). Sequences were aligned, translated, manually edited and separated into V1V2, C2, V3, C3, V4, C4 and V5 subregions. V1-V5 and subregion lengths were calculated, and potential N-linked glycosylation sites (PNLGS) counted. Loop lengths and PNLGS were examined as a function of time since infection, CD4 count, viral load, and calendar year in cross-sectional and longitudinal analyses. V1V2 length and PNLGS increased significantly through chronic infection before declining in late-stage infection. In cross-sectional analyses, V1V2 length also increased by calendar year between 1984 and 2004 in subjects with early and mid-stage illness. Our observations suggest that there is little selection for loop length at the time of transmission; following infection, HIV-1 adapts to host immune responses through increased V1V2 length and/or addition of carbohydrate moieties at N-linked glycosylation sites. V1V2 shortening during early and late-stage infection may reflect ineffective host immunity. Transmission from donors with chronic illness may have caused the modest increase in V1V2 length observed during the course of the pandemic.

Original languageEnglish (US)
Article numbere1001228
JournalPLoS Pathogens
Volume6
Issue number12
DOIs
StatePublished - 2010
Externally publishedYes

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Glycosylation
Disease Progression
HIV-1
env Gene Products
Infection
Cross-Sectional Studies
Pandemics
CD4 Lymphocyte Count
Viral Load
Antibody Formation
Amino Acid Sequence
Immunity
Chronic Disease
Carbohydrates
HIV

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Curlin, M., Zioni, R., Hawes, S. E., Liu, Y., Deng, W., Gottlieb, G. S., ... Mullins, J. I. (2010). Hiv-1 envelope subregion length variation during disease progression. PLoS Pathogens, 6(12), [e1001228]. https://doi.org/10.1371/journal.ppat.1001228

Hiv-1 envelope subregion length variation during disease progression. / Curlin, Marcel; Zioni, Rafael; Hawes, Stephen E.; Liu, Yi; Deng, Wenjie; Gottlieb, Geoffrey S.; Zhu, Tuofu; Mullins, James I.

In: PLoS Pathogens, Vol. 6, No. 12, e1001228, 2010.

Research output: Contribution to journalArticle

Curlin, M, Zioni, R, Hawes, SE, Liu, Y, Deng, W, Gottlieb, GS, Zhu, T & Mullins, JI 2010, 'Hiv-1 envelope subregion length variation during disease progression', PLoS Pathogens, vol. 6, no. 12, e1001228. https://doi.org/10.1371/journal.ppat.1001228
Curlin, Marcel ; Zioni, Rafael ; Hawes, Stephen E. ; Liu, Yi ; Deng, Wenjie ; Gottlieb, Geoffrey S. ; Zhu, Tuofu ; Mullins, James I. / Hiv-1 envelope subregion length variation during disease progression. In: PLoS Pathogens. 2010 ; Vol. 6, No. 12.
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