Histologic changes during selenite cataractogenesis: A light microscopy study

Thomas (Tom) Shearer, R. S. Anderson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this research was to study the early histologic changes occurring in the lens during the formation of selenite overdose cataract. Fourteen-day-old rat pups received a single injection of 2·25 mg Se (kg body wt)-1. Lenses at each of three stages of cataract formation were observed biomicroscopically, and then 1-2 μm sections of methacrylate-embedded lenses were evaluated with the light microscope. The first observable change was vacuolization, which started at the lens bow and later spread throughout the posterior subcapsular region (Stage 1). This was followed by an abrupt increase in basophilia at the interface between the peripheral nucleus and cortex (Stage 2). Bubsequent formation of nuclear opacity was characterized by the presence of opaque particles, abnormal basophilic structures, and lens fiber damage (Stage 3). The data were consistent with the hypothesis that an early site of attack of selenite is at or near the lens bow, and this leads to biochemical changes, fiber cell damage, impaired nutrient circulation, and formation of nuclear cataract.

Original languageEnglish (US)
Pages (from-to)557-565
Number of pages9
JournalExperimental Eye Research
Volume40
Issue number4
DOIs
StatePublished - 1985

Fingerprint

Selenious Acid
Lenses
Microscopy
Light
Cataract
Methacrylates
Food
Injections
Research

Keywords

  • cataract
  • histology
  • lens slit lamp
  • light microscope
  • selenium

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Histologic changes during selenite cataractogenesis : A light microscopy study. / Shearer, Thomas (Tom); Anderson, R. S.

In: Experimental Eye Research, Vol. 40, No. 4, 1985, p. 557-565.

Research output: Contribution to journalArticle

@article{915e57e8042343cb9b2eebfbdd82f90a,
title = "Histologic changes during selenite cataractogenesis: A light microscopy study",
abstract = "The purpose of this research was to study the early histologic changes occurring in the lens during the formation of selenite overdose cataract. Fourteen-day-old rat pups received a single injection of 2·25 mg Se (kg body wt)-1. Lenses at each of three stages of cataract formation were observed biomicroscopically, and then 1-2 μm sections of methacrylate-embedded lenses were evaluated with the light microscope. The first observable change was vacuolization, which started at the lens bow and later spread throughout the posterior subcapsular region (Stage 1). This was followed by an abrupt increase in basophilia at the interface between the peripheral nucleus and cortex (Stage 2). Bubsequent formation of nuclear opacity was characterized by the presence of opaque particles, abnormal basophilic structures, and lens fiber damage (Stage 3). The data were consistent with the hypothesis that an early site of attack of selenite is at or near the lens bow, and this leads to biochemical changes, fiber cell damage, impaired nutrient circulation, and formation of nuclear cataract.",
keywords = "cataract, histology, lens slit lamp, light microscope, selenium",
author = "Shearer, {Thomas (Tom)} and Anderson, {R. S.}",
year = "1985",
doi = "10.1016/0014-4835(85)90077-6",
language = "English (US)",
volume = "40",
pages = "557--565",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Histologic changes during selenite cataractogenesis

T2 - A light microscopy study

AU - Shearer, Thomas (Tom)

AU - Anderson, R. S.

PY - 1985

Y1 - 1985

N2 - The purpose of this research was to study the early histologic changes occurring in the lens during the formation of selenite overdose cataract. Fourteen-day-old rat pups received a single injection of 2·25 mg Se (kg body wt)-1. Lenses at each of three stages of cataract formation were observed biomicroscopically, and then 1-2 μm sections of methacrylate-embedded lenses were evaluated with the light microscope. The first observable change was vacuolization, which started at the lens bow and later spread throughout the posterior subcapsular region (Stage 1). This was followed by an abrupt increase in basophilia at the interface between the peripheral nucleus and cortex (Stage 2). Bubsequent formation of nuclear opacity was characterized by the presence of opaque particles, abnormal basophilic structures, and lens fiber damage (Stage 3). The data were consistent with the hypothesis that an early site of attack of selenite is at or near the lens bow, and this leads to biochemical changes, fiber cell damage, impaired nutrient circulation, and formation of nuclear cataract.

AB - The purpose of this research was to study the early histologic changes occurring in the lens during the formation of selenite overdose cataract. Fourteen-day-old rat pups received a single injection of 2·25 mg Se (kg body wt)-1. Lenses at each of three stages of cataract formation were observed biomicroscopically, and then 1-2 μm sections of methacrylate-embedded lenses were evaluated with the light microscope. The first observable change was vacuolization, which started at the lens bow and later spread throughout the posterior subcapsular region (Stage 1). This was followed by an abrupt increase in basophilia at the interface between the peripheral nucleus and cortex (Stage 2). Bubsequent formation of nuclear opacity was characterized by the presence of opaque particles, abnormal basophilic structures, and lens fiber damage (Stage 3). The data were consistent with the hypothesis that an early site of attack of selenite is at or near the lens bow, and this leads to biochemical changes, fiber cell damage, impaired nutrient circulation, and formation of nuclear cataract.

KW - cataract

KW - histology

KW - lens slit lamp

KW - light microscope

KW - selenium

UR - http://www.scopus.com/inward/record.url?scp=0022336464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022336464&partnerID=8YFLogxK

U2 - 10.1016/0014-4835(85)90077-6

DO - 10.1016/0014-4835(85)90077-6

M3 - Article

C2 - 4007070

AN - SCOPUS:0022336464

VL - 40

SP - 557

EP - 565

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 4

ER -