Highly scalable generation of DNA methylation profiles in single cells

Ryan M. Mulqueen, Dmitry Pokholok, Steven J. Norberg, Kristof A. Torkenczy, Andrew J. Fields, Duanchen Sun, John R. Sinnamon, Jay Shendure, Cole Trapnell, Brian O'Roak, Zheng Xia, Frank J. Steemers, Andrew Adey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We present a highly scalable assay for whole-genome methylation profiling of single cells. We use our approach, single-cell combinatorial indexing for methylation analysis (sci-MET), to produce 3,282 single-cell bisulfite sequencing libraries and achieve read alignment rates of 68 ± 8%. We apply sci-MET to discriminate the cellular identity of a mixture of three human cell lines and to identify excitatory and inhibitory neuronal populations from mouse cortical tissue.

Original languageEnglish (US)
Pages (from-to)428-431
Number of pages4
JournalNature Biotechnology
Volume36
Issue number5
DOIs
StatePublished - Jun 1 2018

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Methylation
DNA Methylation
Assays
Genes
Cells
Tissue
Genome
Cell Line
Population

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Mulqueen, R. M., Pokholok, D., Norberg, S. J., Torkenczy, K. A., Fields, A. J., Sun, D., ... Adey, A. (2018). Highly scalable generation of DNA methylation profiles in single cells. Nature Biotechnology, 36(5), 428-431. https://doi.org/10.1038/nbt.4112

Highly scalable generation of DNA methylation profiles in single cells. / Mulqueen, Ryan M.; Pokholok, Dmitry; Norberg, Steven J.; Torkenczy, Kristof A.; Fields, Andrew J.; Sun, Duanchen; Sinnamon, John R.; Shendure, Jay; Trapnell, Cole; O'Roak, Brian; Xia, Zheng; Steemers, Frank J.; Adey, Andrew.

In: Nature Biotechnology, Vol. 36, No. 5, 01.06.2018, p. 428-431.

Research output: Contribution to journalArticle

Mulqueen, RM, Pokholok, D, Norberg, SJ, Torkenczy, KA, Fields, AJ, Sun, D, Sinnamon, JR, Shendure, J, Trapnell, C, O'Roak, B, Xia, Z, Steemers, FJ & Adey, A 2018, 'Highly scalable generation of DNA methylation profiles in single cells', Nature Biotechnology, vol. 36, no. 5, pp. 428-431. https://doi.org/10.1038/nbt.4112
Mulqueen RM, Pokholok D, Norberg SJ, Torkenczy KA, Fields AJ, Sun D et al. Highly scalable generation of DNA methylation profiles in single cells. Nature Biotechnology. 2018 Jun 1;36(5):428-431. https://doi.org/10.1038/nbt.4112
Mulqueen, Ryan M. ; Pokholok, Dmitry ; Norberg, Steven J. ; Torkenczy, Kristof A. ; Fields, Andrew J. ; Sun, Duanchen ; Sinnamon, John R. ; Shendure, Jay ; Trapnell, Cole ; O'Roak, Brian ; Xia, Zheng ; Steemers, Frank J. ; Adey, Andrew. / Highly scalable generation of DNA methylation profiles in single cells. In: Nature Biotechnology. 2018 ; Vol. 36, No. 5. pp. 428-431.
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