High-throughput screening for identification of novel innate immune activators

Bryan J. Gall, Victor De Filippis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Modern drug discovery has embraced in vitro platforms that enable investigation of large numbers of compounds within tractable timeframes and for feasible costs. These endeavors have been greatly aided in recent years by advances in molecular and cell-based methods such as gene delivery and editing technology, advanced imaging, robotics, and quantitative analysis. As such, the examination of phenotypic impacts of novel molecules may only be limited by the size of the compound collection. Innate immune signaling processes in mammalian cells are especially amenable to high-throughput screening platforms since the cellular responses elicited by their activation often result in high level transcription that can be harnessed in the form of bioluminescent or fluorescent signal. In addition, targeted activation of innate immune pathways represents a valuable therapeutic strategy applicable to multiple chronic and acute human diseases. Herein, we describe the optimization and utilization of a high-throughput screening method using human reporter cells reactive to stimulation of the type I interferon response. Importantly, the principles and methods described can be applied to adherent reporter cells of diverse derivation and innate signaling pathway readouts.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages183-193
Number of pages11
Volume1656
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1656
ISSN (Print)1064-3745

Keywords

  • Drug discovery
  • High-throughput screening
  • Innate immunity
  • Signaling
  • STING

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Gall, B. J., & De Filippis, V. (2017). High-throughput screening for identification of novel innate immune activators. In Methods in Molecular Biology (Vol. 1656, pp. 183-193). (Methods in Molecular Biology; Vol. 1656). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7237-1_12