High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection

Laura E. Fuhrman, Kevin V. Shianna, Alejandro Aballay

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor® as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.

Original languageEnglish (US)
Article numbere2882
JournalPloS one
Volume3
Issue number8
DOIs
StatePublished - Aug 6 2008

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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