High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection

Laura E. Fuhrman, Kevin V. Shianna, Alejandro Aballay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor® as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.

Original languageEnglish (US)
Article numbere2882
JournalPLoS One
Volume3
Issue number8
DOIs
StatePublished - Aug 6 2008
Externally publishedYes

Fingerprint

Pathogens
Sorting
Single Nucleotide Polymorphism
Nucleotide Mapping
Throughput
mutants
sorting
pathogens
Automation
Infection
Polymorphism
infection
Bacteria
Animals
Nucleotides
automation
Phenotype
single nucleotide polymorphism
linkage (genetics)
phenotype

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection. / Fuhrman, Laura E.; Shianna, Kevin V.; Aballay, Alejandro.

In: PLoS One, Vol. 3, No. 8, e2882, 06.08.2008.

Research output: Contribution to journalArticle

@article{39c8210ea14c4b5f87f7632eec2ff247,
title = "High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection",
abstract = "We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor{\circledR} as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.",
author = "Fuhrman, {Laura E.} and Shianna, {Kevin V.} and Alejandro Aballay",
year = "2008",
month = "8",
day = "6",
doi = "10.1371/journal.pone.0002882",
language = "English (US)",
volume = "3",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection

AU - Fuhrman, Laura E.

AU - Shianna, Kevin V.

AU - Aballay, Alejandro

PY - 2008/8/6

Y1 - 2008/8/6

N2 - We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor® as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.

AB - We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor® as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.

UR - http://www.scopus.com/inward/record.url?scp=51449091159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51449091159&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0002882

DO - 10.1371/journal.pone.0002882

M3 - Article

C2 - 18682730

AN - SCOPUS:51449091159

VL - 3

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e2882

ER -