High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population

Phillip Barnette, Rebecca Scholl, Mary Blandford, Linda Ballard, Alexander Tsodikov, Jalene Magee, Susana Williams, Margaret Robertson, Francis Ali-Osman, Richard Lemons, Charles Keller

    Research output: Contribution to journalArticlepeer-review

    40 Scopus citations

    Abstract

    Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. We report a low cost, highly reproducible and specific PCR-based high-throughput assay for genotyping different GSTs designed for use in large clinical trials. In comparison to an alternative genotyping method (single nucleotide extension), the sensitivity and specificity of the high throughput assay was shown to be 92 and 97%, respectively, depending on the source of genomic DNA. Using the high-throughput assay, we demonstrate by multivariate analysis an increased risk of acute lymphoblastic leukemia, glial brain tumors, and osteosarcoma for patients carrying nonnull alleles of GSTM1 and/or GSTT1.

    Original languageEnglish (US)
    Pages (from-to)304-313
    Number of pages10
    JournalCancer Epidemiology Biomarkers and Prevention
    Volume13
    Issue number2
    DOIs
    StatePublished - Feb 2004

    ASJC Scopus subject areas

    • Epidemiology
    • Oncology

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