High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population

Phillip Barnette, Rebecca Scholl, Mary Blandford, Linda Ballard, Alexander Tsodikov, Jalene Magee, Susana Williams, Margaret Robertson, Francis Ali-Osman, Richard Lemons, Charles Keller

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. We report a low cost, highly reproducible and specific PCR-based high-throughput assay for genotyping different GSTs designed for use in large clinical trials. In comparison to an alternative genotyping method (single nucleotide extension), the sensitivity and specificity of the high throughput assay was shown to be 92 and 97%, respectively, depending on the source of genomic DNA. Using the high-throughput assay, we demonstrate by multivariate analysis an increased risk of acute lymphoblastic leukemia, glial brain tumors, and osteosarcoma for patients carrying nonnull alleles of GSTM1 and/or GSTT1.

Original languageEnglish (US)
Pages (from-to)304-313
Number of pages10
JournalCancer Epidemiology Biomarkers and Prevention
Volume13
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

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Glutathione Transferase
Alleles
Pediatrics
Osteosarcoma
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Brain Neoplasms
Neuroglia
Population
Neoplasms
Multivariate Analysis
Nucleotides
Clinical Trials
Costs and Cost Analysis
Drug Therapy
Sensitivity and Specificity
Polymerase Chain Reaction
DNA
Enzymes

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population. / Barnette, Phillip; Scholl, Rebecca; Blandford, Mary; Ballard, Linda; Tsodikov, Alexander; Magee, Jalene; Williams, Susana; Robertson, Margaret; Ali-Osman, Francis; Lemons, Richard; Keller, Charles.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 13, No. 2, 02.2004, p. 304-313.

Research output: Contribution to journalArticle

Barnette, P, Scholl, R, Blandford, M, Ballard, L, Tsodikov, A, Magee, J, Williams, S, Robertson, M, Ali-Osman, F, Lemons, R & Keller, C 2004, 'High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population', Cancer Epidemiology Biomarkers and Prevention, vol. 13, no. 2, pp. 304-313. https://doi.org/10.1158/1055-9965.EPI-03-0178
Barnette, Phillip ; Scholl, Rebecca ; Blandford, Mary ; Ballard, Linda ; Tsodikov, Alexander ; Magee, Jalene ; Williams, Susana ; Robertson, Margaret ; Ali-Osman, Francis ; Lemons, Richard ; Keller, Charles. / High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population. In: Cancer Epidemiology Biomarkers and Prevention. 2004 ; Vol. 13, No. 2. pp. 304-313.
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