High-speed chromosome sorting

Joe Gray, P. N. Dean, J. C. Fuscoe, D. C. Peters, B. J. Trask, G. J. van den Engh, M. A. Van Dilla

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Dual-beam high-speed sorting has been developed to facilitate purification of chromosomes based on DNA staining with the fluorescent dyes Hoechst 33258 and chromomycin A3. Approximately 200 chromosomes per second of two types can be sorted from a suspension of chromosomes isolated from human lymphoblasts while fluorescent objects (chromosomes, debris fragments, chromosome clumps, and nuclei) are processed at the rate of about 20,000 per second. This sorting rate is approximately ten times that possible with conventional sorters. Chormosomes of a single type can be sorted with a purity of about 90 percent. DNA from the sorted chromosomes is suitable for construction of recombinant DNA libraries and for gene mapping.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalScience
Volume238
Issue number4825
StatePublished - Jan 1 1987
Externally publishedYes

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Chromosomes
Chromomycin A3
Bisbenzimidazole
Recombinant DNA
Chromosome Mapping
DNA
Human Chromosomes
Gene Library
Fluorescent Dyes
Suspensions
Staining and Labeling

ASJC Scopus subject areas

  • General

Cite this

Gray, J., Dean, P. N., Fuscoe, J. C., Peters, D. C., Trask, B. J., van den Engh, G. J., & Van Dilla, M. A. (1987). High-speed chromosome sorting. Science, 238(4825), 323-329.

High-speed chromosome sorting. / Gray, Joe; Dean, P. N.; Fuscoe, J. C.; Peters, D. C.; Trask, B. J.; van den Engh, G. J.; Van Dilla, M. A.

In: Science, Vol. 238, No. 4825, 01.01.1987, p. 323-329.

Research output: Contribution to journalArticle

Gray, J, Dean, PN, Fuscoe, JC, Peters, DC, Trask, BJ, van den Engh, GJ & Van Dilla, MA 1987, 'High-speed chromosome sorting', Science, vol. 238, no. 4825, pp. 323-329.
Gray J, Dean PN, Fuscoe JC, Peters DC, Trask BJ, van den Engh GJ et al. High-speed chromosome sorting. Science. 1987 Jan 1;238(4825):323-329.
Gray, Joe ; Dean, P. N. ; Fuscoe, J. C. ; Peters, D. C. ; Trask, B. J. ; van den Engh, G. J. ; Van Dilla, M. A. / High-speed chromosome sorting. In: Science. 1987 ; Vol. 238, No. 4825. pp. 323-329.
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