High specific infectivity of plasma virus from the pre-ramp-up and ramp-up stages of acute simian immunodeficiency virus infection

Zhong Min Ma, Mars Stone, Mike Piatak, Becky Schweighardt, Nancy Haigwood, David Montefiori, Jeffrey D. Lifson, Michael P. Busch, Christopher J. Miller

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    72 Scopus citations

    Abstract

    To define the ratio of simian immunodeficiency virus (SIV) RNA molecules to infectious virions in plasma, a ramp-up-stage plasma pool was made from the earliest viral RNA (vRNA)-positive plasma samples (collected approximately 7 days after inoculation) from seven macaques, and a set-point-stage plasma pool was made from plasma samples collected 10 to 16 weeks after peak viremia from seven macaques; vRNA levels in these plasma pools were determined, and serial 10-fold dilutions containing 1 to 1,500 vRNA copies/ml were made. Intravenous (i.v.) inoculation of a 1-ml aliquot of diluted ramp-up-stage plasma containing 20 vRNA copies infected 2 of 2 rhesus macaques, while for the set-point-stage plasma, i.v. inoculation with 1,500 vRNA copies was needed to transmit infection. Further, when the heat-inactivated set-point-stage plasma pool was mixed with ramp-up-stage virions, infection of inoculated macaques was blocked. Notably, 2 of 2 animals inoculated with 85 ml of a pre-ramp-up plasma pool containing

    Original languageEnglish (US)
    Pages (from-to)3288-3297
    Number of pages10
    JournalJournal of Virology
    Volume83
    Issue number7
    DOIs
    Publication statusPublished - Apr 2009

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    ASJC Scopus subject areas

    • Immunology
    • Virology

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