High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography

Zhongwei Zhi, Yeongri Jung, Jia Yali, Lin An, Ruikang K. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHS-OMAG) for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based on spectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to perform an imaging speed of 150 frames per second that takes only ∼7 seconds to acquire a 3D image. The technique, capable of measuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubular capillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depth-resolved volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature during renal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualized and quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) which relates to abnormal microvasculature.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7898
DOIs
StatePublished - 2011
Externally publishedYes
EventDynamics and Fluctuations in Biomedical Photonics VIII - San Francisco, CA, United States
Duration: Jan 22 2011Jan 24 2011

Other

OtherDynamics and Fluctuations in Biomedical Photonics VIII
CountryUnited States
CitySan Francisco, CA
Period1/22/111/24/11

Fingerprint

Microcirculation
ischemia
Kidney
Imaging techniques
kidney diseases
glomerulus
cortexes
Optical tomography
blood flow
CCD cameras
imaging techniques
Imaging systems
Microvessels
Labels
Blood
tomography
Ischemia
Optical Imaging
Optical Coherence Tomography
Chronic Renal Insufficiency

Keywords

  • Chronic kidney disease (CKD)
  • Optical coherence tomography (OCT)
  • Optical microangiography (OMAG)
  • Renal microcirculation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Zhi, Z., Jung, Y., Yali, J., An, L., & Wang, R. K. (2011). High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7898). [789816] https://doi.org/10.1117/12.875694

High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. / Zhi, Zhongwei; Jung, Yeongri; Yali, Jia; An, Lin; Wang, Ruikang K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7898 2011. 789816.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhi, Z, Jung, Y, Yali, J, An, L & Wang, RK 2011, High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7898, 789816, Dynamics and Fluctuations in Biomedical Photonics VIII, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.875694
Zhi Z, Jung Y, Yali J, An L, Wang RK. High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7898. 2011. 789816 https://doi.org/10.1117/12.875694
Zhi, Zhongwei ; Jung, Yeongri ; Yali, Jia ; An, Lin ; Wang, Ruikang K. / High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7898 2011.
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