High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography

Zhongwei Zhi, Yeongri Jung, Yali Jia, Lin An, Ruikang K. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHS-OMAG) for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based on spectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to perform an imaging speed of 150 frames per second that takes only ∼7 seconds to acquire a 3D image. The technique, capable of measuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubular capillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depth-resolved volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature during renal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualized and quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) which relates to abnormal microvasculature.

Original languageEnglish (US)
Title of host publicationDynamics and Fluctuations in Biomedical Photonics VIII
DOIs
StatePublished - Apr 14 2011
EventDynamics and Fluctuations in Biomedical Photonics VIII - San Francisco, CA, United States
Duration: Jan 22 2011Jan 24 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7898
ISSN (Print)1605-7422

Other

OtherDynamics and Fluctuations in Biomedical Photonics VIII
CountryUnited States
CitySan Francisco, CA
Period1/22/111/24/11

Keywords

  • Chronic kidney disease (CKD)
  • Optical coherence tomography (OCT)
  • Optical microangiography (OMAG)
  • Renal microcirculation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography'. Together they form a unique fingerprint.

  • Cite this

    Zhi, Z., Jung, Y., Jia, Y., An, L., & Wang, R. K. (2011). High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography. In Dynamics and Fluctuations in Biomedical Photonics VIII [789816] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7898). https://doi.org/10.1117/12.875694