TY - GEN
T1 - High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography
AU - Zhi, Zhongwei
AU - Jung, Yeongri
AU - Jia, Yali
AU - An, Lin
AU - Wang, Ruikang K.
PY - 2011
Y1 - 2011
N2 - We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHS-OMAG) for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based on spectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to perform an imaging speed of 150 frames per second that takes only ∼7 seconds to acquire a 3D image. The technique, capable of measuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubular capillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depth-resolved volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature during renal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualized and quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) which relates to abnormal microvasculature.
AB - We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHS-OMAG) for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based on spectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to perform an imaging speed of 150 frames per second that takes only ∼7 seconds to acquire a 3D image. The technique, capable of measuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubular capillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depth-resolved volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature during renal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualized and quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) which relates to abnormal microvasculature.
KW - Chronic kidney disease (CKD)
KW - Optical coherence tomography (OCT)
KW - Optical microangiography (OMAG)
KW - Renal microcirculation
UR - http://www.scopus.com/inward/record.url?scp=79953810197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953810197&partnerID=8YFLogxK
U2 - 10.1117/12.875694
DO - 10.1117/12.875694
M3 - Conference contribution
AN - SCOPUS:79953810197
SN - 9780819484352
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Dynamics and Fluctuations in Biomedical Photonics VIII
T2 - Dynamics and Fluctuations in Biomedical Photonics VIII
Y2 - 22 January 2011 through 24 January 2011
ER -