High-resolution 23Na-NMR studies of human erythrocytes: Use of aqueous shift reagents

M. M. Pike, E. T. Fossel, T. W. Smith, C. S. Springer

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Aqueous shift reagents were used to clearly distinguish intra- and extracellular 23Na-nuclear magnetic resonance (NMR) signals in samples consisting of whole blood or suspensions of washed human erythrocytes (both fresh and outdated). The lanthanide chelates Dy(PPP)27- and Tm(TTHA)3- were used to shift the extracellular signals upfield, and Dy(TTHA)3- and Tm(PPP)27- were similarly used to shift extracellular resonance downfield. The absolute intensities of the signals were used along with the measured hematocrit to simultaneously determine the intra- and extracellular Na+ concentrations. The results were generally within 5% of the values determined by more time-consuming centrifugation-flame emission photometry measurements on the same samples. Thus the 23Na-NMR signals from both intra- and extracellular cations suffer no NMR invisibility within experimental error. The lower level of intracellular Na+ in fresh erythrocytes (<12 mM) is easily distinguished from the higher level (~30 mM) in erythrocytes that have been stored (in the cold) outside the body for some weeks.

Original languageEnglish (US)
Pages (from-to)C528-C536
JournalAmerican Journal of Physiology - Cell Physiology
Volume15
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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