High resolution chromosome analysis

one and two parameter flow cytometry

Joe Gray, R. G. Langlois, A. V. Carrano, K. Burkhart-Schulte, M. A. Van Dilla

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Isolated mammalian chromosomes have been quantitatively classified by high resolution flow cytometry. Chinese hamster chromosomes stained with 33258 Hoechst and excited in the UV showed a fluorescence distribution in which the 14 types of Chinese hamster chromosomes were resolved into 16 groups seen as distinct peaks in the distributions. Chinese hamster chromosomes were also stained with both 33258 Hoechst (HO) and chromomycin A3 (CA3); the two dye contents were measured by selective excitation in the UV and at 458 nm in a dual beam flow cytometer. The resulting two parameter distribution (HO versus CA3) showed 10 chromosome groups1. Human strain LLL 761 chromosomes stained with HO and excited in the UV showed a fluorescence distribution in which the 23 types of human chromosomes were resolved into 12 groups. Human chromosomes stained with both HO and CA3 and measured in the dual beam flow cytometer produced two parameter fluorescence distributions which showed 20 groups. The chromosomes associated with each group were determined by quinacrine banding analysis of sorted chromosomes and by DNA cytophotometry of preidentified metaphase chromosomes. The relative HO and CA3 stain content and frequency of occurrence of chromosomes in each group were determined from the fluorescence distributions and compared to the results from DNA cytophotometry. The chromosome to chromosome variations in HO and CA3 staining are attributed to variations in chromosomal base composition.

Original languageEnglish (US)
Pages (from-to)9-27
Number of pages19
JournalChromosoma
Volume73
Issue number1
DOIs
StatePublished - Jan 1979
Externally publishedYes

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Flow Cytometry
Chromomycin A3
Chromosomes
Cricetulus
Fluorescence
Cytophotometry
Bisbenzimidazole
Human Chromosomes
Coloring Agents
Mammalian Chromosomes
Quinacrine
Chromosomes, Human, Pair 10
DNA
Base Composition
Metaphase
Staining and Labeling

ASJC Scopus subject areas

  • Genetics

Cite this

Gray, J., Langlois, R. G., Carrano, A. V., Burkhart-Schulte, K., & Van Dilla, M. A. (1979). High resolution chromosome analysis: one and two parameter flow cytometry. Chromosoma, 73(1), 9-27. https://doi.org/10.1007/BF00294840

High resolution chromosome analysis : one and two parameter flow cytometry. / Gray, Joe; Langlois, R. G.; Carrano, A. V.; Burkhart-Schulte, K.; Van Dilla, M. A.

In: Chromosoma, Vol. 73, No. 1, 01.1979, p. 9-27.

Research output: Contribution to journalArticle

Gray, J, Langlois, RG, Carrano, AV, Burkhart-Schulte, K & Van Dilla, MA 1979, 'High resolution chromosome analysis: one and two parameter flow cytometry', Chromosoma, vol. 73, no. 1, pp. 9-27. https://doi.org/10.1007/BF00294840
Gray J, Langlois RG, Carrano AV, Burkhart-Schulte K, Van Dilla MA. High resolution chromosome analysis: one and two parameter flow cytometry. Chromosoma. 1979 Jan;73(1):9-27. https://doi.org/10.1007/BF00294840
Gray, Joe ; Langlois, R. G. ; Carrano, A. V. ; Burkhart-Schulte, K. ; Van Dilla, M. A. / High resolution chromosome analysis : one and two parameter flow cytometry. In: Chromosoma. 1979 ; Vol. 73, No. 1. pp. 9-27.
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