High-resolution 23Na-NMR studies of human erythrocytes: Use of aqueous shift reagents

Martin Pike, E. T. Fossel, T. W. Smith, Charles Jr Springer

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aqueous shift reagents were used to clearly distinguish intra- and extracellular 23Na-nuclear magnetic resonance (NMR) signals in samples consisting of whole blood or suspensions of washed human erythrocytes (both fresh and outdated). The lanthanide chelates Dy(PPP)2 7- and Tm(TTHA)3- were used to shift the extracellular signals upfield, and Dy(TTHA)3- and Tm(PPP)2 7- were similarly used to shift extracellular resonance downfield. The absolute intensities of the signals were used along with the measured hematocrit to simultaneously determine the intra- and extracellular Na+ concentrations. The results were generally within 5% of the values determined by more time-consuming centrifugation-flame emission photometry measurements on the same samples. Thus the 23Na-NMR signals from both intra- and extracellular cations suffer no NMR invisibility within experimental error. The lower level of intracellular Na+ in fresh erythrocytes (

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume15
Issue number3
StatePublished - 1984
Externally publishedYes

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Magnetic Resonance Spectroscopy
Erythrocytes
Nuclear magnetic resonance
Photometry
Lanthanoid Series Elements
Centrifugation
Hematocrit
Cations
Suspensions
Blood
triethylenetetraminehexaacetic acid
preclamol

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

High-resolution 23Na-NMR studies of human erythrocytes : Use of aqueous shift reagents. / Pike, Martin; Fossel, E. T.; Smith, T. W.; Springer, Charles Jr.

In: American Journal of Physiology - Cell Physiology, Vol. 15, No. 3, 1984.

Research output: Contribution to journalArticle

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